Applied Microbiology and Biotechnology

, Volume 70, Issue 2, pp 254–259 | Cite as

A novel crude oil emulsifier excreted in the culture supernatant of a marine bacterium, Myroides sp. strain SM1

  • Suppasil Maneerat
  • Takeshi Bamba
  • Kazuo Harada
  • Akio Kobayashi
  • Hidenori Yamada
  • Fusako Kawai
Applied Microbial and Cell Physiology


A marine bacterium, Myroides sp. SM1, can grow on weathered crude oil and show emulsification of it. The biosurfactant able to emulsify crude oil was excreted in culture supernatant of Myroides sp. SM1 grown on marine broth, which was extracted with chloroform/methanol (1:1) at pH 7 and purified by normal and reverse phase silica gel column chromatographies. The compound was ninhydrin-positive, and the chemical structure was elucidated by nuclear magnetic resonance (NMR), infrared spectroscopy (IR), fast atom bombardment mass spectrometry, and gas chromatography–mass spectrometry (GC-MS) to be a mixture of l-ornithine lipids, which were composed of l-ornithine and a different couple of iso-3-hydroxyfatty acid (C15–C17) and iso-fatty acid (C15 or C16) in a ratio of 1:1:1. The critical micelle concentration for a mixture of ornithine lipids was measured to be approximately 40 mg/l. A mixture of ornithine lipids exhibited emulsifying activity for crude oil in a broad range of pH, temperature, and salinity and showed higher surface activity for oil displacement test than other several artificial surfactants and a biosurfactant, surfactin.


Biosurfactants Surfactin Rhamnolipids Lipopeptide Biosurfactants Emulsification Ability 
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We are grateful to the JSPS-NRCT Core University Program for providing a scholarship to S. M.; to the Marine Biotechnology Institute, Iwate, Japan, for their kind supply of weathered crude oil; and to Mr. A. Nara (Termo Electron Co.) for FT-IR analysis. We appreciate financial supports to F. K. by Nihonseimei Foundation. NMR and FAB-MS spectra were measured at the Analytical Center of Engineering, Osaka University.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Suppasil Maneerat
    • 1
  • Takeshi Bamba
    • 2
  • Kazuo Harada
    • 2
  • Akio Kobayashi
    • 2
  • Hidenori Yamada
    • 3
  • Fusako Kawai
    • 1
  1. 1.Laboratory of Applied Microbiology, Research Institute for BioresourcesOkayama UniversityOkayamaJapan
  2. 2.Department of Biotechnology, Graduate School of EngineeringOsaka UniversityOsakaJapan
  3. 3.Department of Bioscience and Biotechnology, Faculty of EngineeringOkayama UniversityOkayamaJapan

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