Applied Microbiology and Biotechnology

, Volume 70, Issue 1, pp 47–52 | Cite as

Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor

  • Kazuichi IsakaEmail author
  • Yasuhiro Date
  • Tatsuo Sumino
  • Sachiko Yoshie
  • Satoshi Tsuneda
Biotechnological Products and Process Engineering


The doubling time of anaerobic ammonium-oxidizing (anammox) bacteria in an anaerobic biological filtrated (ABF) reactor was determined. Fluorescence in situ hybridization analysis was used to detect and count anammox bacteria cells in anammox sludge. As a result, the populations of anammox bacteria at 14th and 21st days were 1.1×106 and 1.7×107 cells/ml reactor, respectively. From these results, the doubling time of anammox bacteria was calculated as 1.8 days, and the specific growth rate (μ) was 0.39 day−1. This result indicated that the anammox bacteria have higher growth rate than the reported value (doubling time, 11 days). Furthermore, it was clearly demonstrated that nitrogen conversion rate was proportional to the population of anammox bacteria. Maintaining the ideal environment for the growth of anammox bacteria in the ABF reactor might lead to faster growth. This is the first report of the growth rate of anammox bacteria based on the direct counting of anammox bacteria.


Sludge Anammox Nitrogen Removal Sequencing Batch Reactor Anammox Bacterium 
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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Kazuichi Isaka
    • 1
    Email author
  • Yasuhiro Date
    • 2
  • Tatsuo Sumino
    • 1
  • Sachiko Yoshie
    • 2
  • Satoshi Tsuneda
    • 2
  1. 1.Hitachi Plant Engineering and Construction Co., Ltd.MatsudoJapan
  2. 2.Department of Chemical EngineeringWaseda UniversityTokyoJapan

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