Applied Microbiology and Biotechnology

, Volume 69, Issue 5, pp 515–520 | Cite as

Microbial production of single-cell protein from deproteinized whey concentrates

  • Nadja Schultz
  • Lifung Chang
  • Achim Hauck
  • Matthias Reuss
  • Christoph Syldatk
Biotechnological Products and Process Engineering

Abstract

Deproteinized sweet and sour cheese whey concentrates were investigated for their suitability as substrates for the production of single-cell protein with Kluyveromyces marxianus CBS 6556 up to a 100-l scale. An important factor for gaining high cell concentrations was the use of the Crabtree-negative strain K. marxianus CBS 6556. Supplements such as trace elements, ammonium and calcium were required for the complete conversion of sweet whey concentrates into biomass, whereas sour whey concentrates had to be supplemented with ammonium, trace elements and vitamins. After improvement, biomass dry concentrations of up to 50 g l−1 could be reached with Yx/s values of 0.52 for sweet whey and of up to 65 g l−1 with Yx/s values of 0.48 for sour whey concentrates. The chemical oxygen demand of the whey concentrates were reduced by 80%. The cells were used for the analysis of amino acid and ash composition, showing a distinct increase of eight out of ten essential amino acids compared to sweet and sour whey protein and exceeding the World Health Organisation guidelines for valine, leucine, isoleucine, threonine, phenylalanine and tyrosine.

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Copyright information

© Springer 2005

Authors and Affiliations

  • Nadja Schultz
    • 1
    • 2
  • Lifung Chang
    • 1
    • 3
  • Achim Hauck
    • 1
  • Matthias Reuss
    • 1
  • Christoph Syldatk
    • 1
    • 2
  1. 1.Universität StuttgartInstitut für BioverfahrenstechnikStuttgartGermany
  2. 2.Engler-Bunte-Institut, Lehrstuhl für Technische BiologieUniversity of Karlsruhe (TH)KarlsruheGermany
  3. 3.Pall Filtration Pte LtdTaipeiTaiwan

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