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Applied Microbiology and Biotechnology

, Volume 67, Issue 3, pp 383–388 | Cite as

Adaptation of Rhodococcus erythropolis DCL14 to growth on n-alkanes, alcohols and terpenes

  • Carla C. C. R. de Carvalho
  • Beatriz Parreño-Marchante
  • Grit Neumann
  • M. Manuela R. da Fonseca
  • Hermann J. Heipieper
Applied Microbial and Cell Physiology

Abstract

Rhodococcus erythropolis DCL14 has the ability to convert the terpene (−)-carveol to the valuable flavour compound (−)-carvone when growing on a wide range of carbon sources. To study the effect of carbon and energy sources such as alkanes, alkanols and terpenes on the biotechnological process, the cellular adaptation at the level of fatty acid composition of the membrane phospholipids and the (−)-carvone production were examined. All tested carbon sources caused a dose-dependent increase in the degree of saturation of the fatty acids. The exception was observed with short-chain alcohols such as methanol and ethanol, to which the cells adapted with a concentration-dependent decrease in the saturation degree of the membrane phospholipids. This influence of the different carbon sources on the rigidity of the cell membrane also had an impact on the (−)-carvone productivity of the strain.

Keywords

Terpene Limonene Carvone Membrane Fatty Acid Mycolic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This study was supported by a post-doctoral grant (SFRH/BPD/14426/2003) awarded to C.C.C.R.C. by the Fundação para a Ciência e a Tecnologia, Portugal.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Carla C. C. R. de Carvalho
    • 1
  • Beatriz Parreño-Marchante
    • 1
    • 2
  • Grit Neumann
    • 3
  • M. Manuela R. da Fonseca
    • 1
  • Hermann J. Heipieper
    • 3
  1. 1.Centre for Biological and Chemical EngineeringInstituto Superior TécnicoLisboaPortugal
  2. 2.Erasmus student, Facultad de QuímicaUniversidad de MurciaMurciaSpain
  3. 3.Department of BioremediationUFZ-Centre for Environmental Research Leipzig-HalleLeipzigGermany

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