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Applied Microbiology and Biotechnology

, Volume 66, Issue 6, pp 668–674 | Cite as

A Gluconobacter oxydans mutant converting glucose almost quantitatively to 5-keto-d-gluconic acid

  • Mustafa Elfari
  • Seung-Wook Ha
  • Christoph Bremus
  • Marcel Merfort
  • Viola Khodaverdi
  • Ute Herrmann
  • Hermann Sahm
  • Helmut GörischEmail author
Applied Genetics and Molecular Biotechnology

Abstract

Gluconobacter oxydans converts glucose to gluconic acid and subsequently to 2-keto-d-gluconic acid (2-KGA) and 5-keto-d-gluconic acid (5-KGA) by membrane-bound periplasmic pyrroloquinoline quinone-dependent and flavin-dependent dehydrogenases. The product pattern obtained with several strains differed significantly. To increase the production of 5-KGA, which can be converted to industrially important l-(+)-tartaric acid, growth parameters were optimized. Whereas resting cells of G. oxydans ATCC 621H converted about 11% of the available glucose to 2-KGA and 6% to 5-KGA, with growing cells and improved growth under defined conditions (pH 5, 10% pO2, 0.05% pCO2) a conversion yield of about 45% 5-KGA from the available glucose was achieved. As the accumulation of the by-product 2-KGA is highly disadvantageous for an industrial application of G. oxydans, a mutant was generated in which the membrane-bound gluconate-2-dehydrogenase complex was inactivated. This mutant, MF1, grew in a similar way to the wild type, but formation of the undesired 2-KGA was not observed. Under improved growth conditions, mutant MF1 converted the available glucose almost completely (84%) into 5-KGA. Therefore, this newly developed recombinant strain is suitable for the industrial production of 5-KGA.

Keywords

Gluconic Acid Miglitol Triparental Mating Pyrroloquinoline Quinone Ketogluconic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The project was carried out within the framework of the Competence Network Göttingen “Genome research on bacteria” (GenoMik) financed by the German Federal Ministry of Education and Research (BMBF).

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mustafa Elfari
    • 1
  • Seung-Wook Ha
    • 1
  • Christoph Bremus
    • 2
  • Marcel Merfort
    • 2
  • Viola Khodaverdi
    • 1
  • Ute Herrmann
    • 2
  • Hermann Sahm
    • 2
  • Helmut Görisch
    • 1
    Email author
  1. 1.Fachgebiet Technische BiochemieInstitut für Biotechnologie der Technischen Universität BerlinBerlinGermany
  2. 2.Institut für Biotechnologie1Forschungszentrum Jülich GmbHJülichGermany

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