Applied Microbiology and Biotechnology

, Volume 66, Issue 3, pp 253–258 | Cite as

d-Hexosaminate production by oxidative fermentation

  • D. Moonmangmee
  • O. Adachi
  • H. Toyama
  • K. Matsushita
Applied Microbial and Cell Physiology
First Online:
Received:
Revised:
Accepted:

Abstract

Microbial production of d-hexosaminate was examined by means of oxidative fermentation with acetic acid bacteria. In most strains of acetic acid bacteria, membrane-bound d-glucosamine dehydrogenase (synonymous with an alternative d-glucose dehydrogenase distinct from quinoprotein d-glucose dehydrogenase) oxidized d-hexosamines to the corresponding d-hexosaminates in a stoichiometric manner. Conversion of d-hexosamines to the corresponding d-hexosaminates was observed with growing cells of acetic acid bacteria, and d-hexosaminate was stably accumulated in the culture medium even though d-hexosamine was exhausted. Since the enzyme responsible is located on the outer surface of the cytoplasmic membrane, and the enzyme activity is linked to the respiratory chain of the organisms, resting cells, dried cells, and immobilized cells of acetic acid bacteria were effective catalysts for d-hexosaminate production. d-Mannosaminate and d-galactosaminate were also prepared for the first time by means of oxidative fermentation, and three different d-hexosaminates were isolated from unreacted substrate by a chromatographic separation. In this paper, d-hexosaminate production by oxidative fermentation carried out mainly with Gluconobacter frateurii IFO 3264 is exemplified as a typical example.

Keywords

Immobilize Cell Acetic Acid Bacterium DCIP Pyrroloquinoline Quinone Oxidative Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

The generous donation of an ion exchanging resin, UBK555, by Mitsubishi Chemical, Tokyo, Japan. is greatly acknowledged. A part of this work was done in collaboration with the Core University Program between Yamaguchi University and Kasetsart University, supported by the Scientific Cooperation Program of the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT). The authors are grateful to Dr. R. Iwamoto, Nara Women’s University for her kind help in enzyme purification of d-glucosaminate dehydratase from P. fluorescens.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • D. Moonmangmee
    • 1
  • O. Adachi
    • 2
  • H. Toyama
    • 2
  • K. Matsushita
    • 2
  1. 1.Department of Microbiology, Faculty of ScienceKing Mongkut’s University of Technology ThonburiBangkokThailand
  2. 2.Department of Biological Chemistry, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan

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