Applied Microbiology and Biotechnology

, Volume 66, Issue 1, pp 115–122

Analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (AT-SBR) treating swine waste

  • P. Juteau
  • D. Tremblay
  • R. Villemur
  • J.-G. Bisaillon
  • R. Beaudet
Environmental Biotechnology

Abstract

The microflora of a self-heating aerobic thermophilic sequencing batch reactor (AT-SBR) treating swine waste was investigated by a combination of culture and culture-independent techniques. The temperature increased quickly in the first hours of the treatment cycles and values up to 72°C were reached. Denaturing gradient gel electrophoresis of the PCR-amplified V3 region of 16S rDNA (PCR-DGGE) revealed important changes in the bacterial community during 3-day cycles. A clone library was constructed with the near-full-length 16S rDNA amplified from a mixed-liquor sample taken at 60°C. Among the 78 non-chimeric clones analysed, 20 species (here defined as clones showing more than 97% sequence homology) were found. In contrast to other culture-independent bacterial analyses of aerobic thermophilic wastewater treatments, species belonging to the Bacilli class were dominant (64%) with Bacillus thermocloacae being the most abundant species (38%). The other Bacilli could not be assigned to a known species. Schineria larvae was the second most abundant species (14%) in the clone library. Four species were also found among the 19 strains isolated, cultivated and identified from samples taken at 40°C and 60°C. Ten isolates showed high 16S rDNA sequence homology with the dominant bacterium of a composting process that had not been previously isolated.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • P. Juteau
    • 1
  • D. Tremblay
    • 1
  • R. Villemur
    • 1
  • J.-G. Bisaillon
    • 1
  • R. Beaudet
    • 1
  1. 1.INRS-Institut Armand-FrappierUniversité du QuébecLavalCanada
  2. 2.AgribioticsCambridgeCanada

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