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Applied Microbiology and Biotechnology

, Volume 65, Issue 2, pp 143–148 | Cite as

The effects of extracellular polymeric substances on the formation and stability of biogranules

  • Yong-Qiang Liu
  • Yu LiuEmail author
  • Joo-Hwa Tay
Mini-Review

Abstract

Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aims to review the role of EPS in biogranulation, factors influencing EPS production, the effect of EPS on cell surface properties of biogranules, and the relationship of EPS to the structural stability of biogranules. EPS production is substantially enhanced when the microbial community is subject to stressful culture conditions, and the stimulated EPS production in the microbial matrix in turn favours the formation of anaerobic and aerobic granules. EPS can also play an essential role in maintaining the integrity and stability of spatial structure in mature biogranules. It is expected that this paper can provide deep insights into the functions of EPS in the biogranulation process.

Keywords

Extracellular Polymeric Substance Sequencing Batch Reactor Granular Sludge Extracellular Polysaccharide Upflow Anaerobic Sludge Blanket 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Andreadakis AD (1993) Physical and chemical properties of activated sludge flocs. Water Res 27:1701–1714Google Scholar
  2. Azeredo J, Lazarova V, Oliverira R (1999) Methods to extract the exopolymeric matrix from biofilms: a comparative study. Water Sci Technol 39:243–250CrossRefGoogle Scholar
  3. Babcock GT, Wikstrom M (1992) Oxygen activation and the conservation of energy in cell respiration. Nature 356:301–309CrossRefPubMedGoogle Scholar
  4. Batstone DJ, Keller J (2001) Variation of bulk properties of anaerobic granules with wastewater type. Water Res 35:1723–1729CrossRefPubMedGoogle Scholar
  5. Beer D de, Flaharty VO, Thareesri J, Lens P, Verstraete W (1996) Distribution of extracellular polysaccharides and flotation of anaerobic sludge. Appl Microbiol Biotechnol 46:197–201CrossRefGoogle Scholar
  6. Cammarota MC, Sant’Anna GL (1998) Metabolic blocking of exopolysaccharides synthesis: effects on microbial adhesion and biofilm accumulation. Biotechnol Lett 20:1–4CrossRefGoogle Scholar
  7. Chan CS, De Stasio G, Welch SA, Girasole M, Frazer BH, Nesterova MV, Fakra S, Banfield JF (2004) Microbial polysaccharides template assembly of nanocrystal fibers. Science 303:1656–1658CrossRefPubMedGoogle Scholar
  8. Costerton JW, Irvin RT, Cheng KJ (1981) The bacterial glycocalyx in nature and disease. Annu Rev Microbiol 35:299–324PubMedGoogle Scholar
  9. Daffochio D, Thaveesri J, Verstraete W (1995) Contact angle measurement and cell hydrophobicity of granular sludge from upflow anaerobic sludge bed reactors. Biotechnol Lett 61:3676–3680Google Scholar
  10. Dignac MF, Urbain V, Rybacki D, Bruchet A, Snidaro D, Scribe P (1998) Chemical description of extracellular polymers: implication on activated sludge floc structure. Water Sci Technol 38:45–53CrossRefGoogle Scholar
  11. Durmaz B, Sanin FD (2001) Effect of carbon to nitrogen ratio on the composition of microbial extracellular polymers in activated sludge. Water Sci Technol 44:221–229Google Scholar
  12. El-Mamouni R, Leduc R, Costerton JW, Guiot SR (1995) Influence of the microbial content of different precursory nuclei on the anaerobic granulation dynamics. Water Sci Technol 32:173–177CrossRefGoogle Scholar
  13. Etchebehere C, Cabezas A, Dabert P, Muxi L (2003) Evolution of the bacterial community during granules formation in denitrifying reactors followed by molecular, culture-independent techniques. Water Sci Technol 48:75–79Google Scholar
  14. Fang HHP (2000) Microbial distribution in UASB granules and its resulting effects. Water Sci Technol 42:201–208Google Scholar
  15. Fang HHP, Liu H, Zhang T (2002) Characterization of a hydrogen-producing granular sludge. Biotechnol Bioeng 78:44–52CrossRefPubMedGoogle Scholar
  16. Forster CF (1992) Anaerobic upflow sludge blanket reactors: aspects of their microbiology and chemistry. J Biotechnol 17:221–232CrossRefGoogle Scholar
  17. Frolund B, Palmgren R, Keiding K, Nielsen P (1996) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30:1749–1758CrossRefGoogle Scholar
  18. Fukuzaki S, Nishio N, Nagai S (1995) High rate performance and characterisation of granular methanogenic sludges in upflow anaerobic sludge blanket reactors fed with various defined substrates. J Ferment Bioeng 79:354–359CrossRefGoogle Scholar
  19. Ghigo JM (2003) Are there biofilm-specific physiological pathways beyond a reasonable doubt? Res Microbiol 154:1–8CrossRefPubMedGoogle Scholar
  20. Goodwin JAS, Forster CF (1985) A further examination into the composition of activated sludge surfaces in relation to their settlement characteristics. Water Res 19:527–533CrossRefGoogle Scholar
  21. Grotenhuis JTC, Smith M, van Lammeran AAM, Stams AJM, Zehnder AJB (1991) Localization and quantification of extracellular polymers in methanogenic granular sludge. Appl Microbiol Biotechnol 36:115–119Google Scholar
  22. Horan NJ, Eccles CR (1986) Purification and characterization of extracellular polysaccharide from activated sludge. Water Res 20:1427–1432CrossRefGoogle Scholar
  23. Jiang HL, Tay JH, Tay STL (2002) Aggregation of immobilized activated sludge into aerobically grown microbial granule for the aerobic biodegradation of phenol. Lett Appl Microbiol 35:439–445CrossRefPubMedGoogle Scholar
  24. Jiang HL, Tay JH, Tay STL (2003) Changes in structure, activity and metabolism of aerobic granules as a microbial response to high phenol loading. Appl Microbiol Biotechnol 63:602–608CrossRefPubMedGoogle Scholar
  25. Jorand F, Guicherd P, Urbain V, Manem J, Block JC (1994) Hydrophobicity of activated sludge flocs and laboratory-growth bacteria. Water Sci Technol 30:211–218Google Scholar
  26. Jorand F, Zartarian F, Thomas F, Block JC, Bottero JY, Villemin G, Urbain V, Manem J (1995) Chemical and structural (2D) linkage between bacteria within activated sludge flocs. Water Res 29:1639–1647CrossRefGoogle Scholar
  27. Jorand F, Boue-Bigne F, Block JC, Urbain V (1998) Hydrophobic/hydrophilic properties of activated sludge exopolymeric substances. Water Sci Technol 37:307–315CrossRefGoogle Scholar
  28. Liao BQ, Allen DG, Droppo IG, Leppard GG, Liss SN (2001) Surface properties of sludge and their role in bioflocculation and settleability. Water Res 35:339–350CrossRefPubMedGoogle Scholar
  29. Liu H, Fang HHP (2002) Characterization of electrostatic binding sites of extracellular polymers by linear programming analysis of titration data. Biotechnol Bioeng 80:806–811CrossRefPubMedGoogle Scholar
  30. Liu Y, Xu HL, Show KY, Tay JH (2002) Anaerobic granulation technology for wastewater treatment. World J Microbiol Biotechnol 18:99–113CrossRefGoogle Scholar
  31. Liu QS, Tay JH, Liu Y (2003) Substrate concentration-independent aerobic granulation in sequential aerobic sludge blanket reactor. Environ Technol 24:1235–1242PubMedGoogle Scholar
  32. Liu Y, Yang SF, Tay JH (2004a) Improved stability of aerobic granules by selecting slow-growing nitrifying bacteria. J Biotechnol 108:161–169CrossRefPubMedGoogle Scholar
  33. Liu Y, Yang SF, Tay JH, Liu QS, Qin Lei, Li Y (2004b) Cell hydrophobicity is a triggering force of biogranulation. Enzyme Microb Technol 34:371–379CrossRefGoogle Scholar
  34. Lopez JM, Koopman B, Bitton G (1986) INT-dehydrogenase test for activated sludge process control. Biotechnol Bioeng 28:1080–1085Google Scholar
  35. Macleod FA, Guiot SR, Costerton JW (1995) Electron-microscopic examination of the extracellular polymeric substances in anaerobic granular biofilms. World J Microbiol Biotechnol 11:481–485Google Scholar
  36. Mahmoud N, Zeeman G, Gijzen H, Lettinga G (2003) Solids removal in upflow anaerobic reactors, a review. Bioresour Technol 90:1–9CrossRefPubMedGoogle Scholar
  37. Martinez FO, Lema J, Mendez R, Cuervo-Lopez F, Gomez J (2004) Role of exopolymeric protein on the settleability of nitrifying sludges. Bioresour Technol 94:43–48CrossRefPubMedGoogle Scholar
  38. Mcnab R, Forbes H, Handley P, Loach DM, Tannock GW, Jenkison HF (1999) Cell wall-anchored CshA polypeptide (259 kilodaltons) in Streptococcus gordonii forms surface fibrils that confer hydrophobic and adhesive properties. J Bacteriol 181:3087–3095PubMedGoogle Scholar
  39. Morgan JW, Forster CF, Evison L (1990) A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Res 24:743–750CrossRefGoogle Scholar
  40. Nichols CAM, Garon S, Bowman JP, Raguenes G, Guezennec J (2004) Production of exopolysaccharides by Antarctic marine bacterial isolates. J Appl Microbiol 96:1057–1066CrossRefPubMedGoogle Scholar
  41. Nielsen PH, Jahn A, Palmgren R (1997) Conceptual model for production and composition of exopolymers in biofilms. Water Sci Technol 36:11–19CrossRefGoogle Scholar
  42. Pavoni JL, Tenney MW, Echelberger JWF (1972) Bacterial extracellular polymers and biological flocculation. J Water Pollut Control Fed 44:414–431PubMedGoogle Scholar
  43. Punal A, Trevisan M, Rozzi A, Lema JM (2000) Influence of C:N ratio on the start-up of up-flow anaerobic filter reactors. Water Res 34:2614–2619CrossRefGoogle Scholar
  44. Punal A, Brauchi S, Rdyes JG, Chamy R (2003) Dynamics of extracellular polymeric substances in UASB and EGSB reactors treating medium and low concentrated wastewaters. Water Sci Technol 48:41–49PubMedGoogle Scholar
  45. Qin L, Liu QS, Yang SF, Tay JH, Liu Y (2004a) Stressful conditions-induced production of extracellular polysaccharides in aerobic granulation process. Civil Eng Res 17:49–51Google Scholar
  46. Qin L, Tay JH, Liu Y (2004b) Selection pressure is a driving force of aerobic granulation in sequencing batch reactors. Process Biochem 39:579–584CrossRefGoogle Scholar
  47. Quarmby J, Forster CF (1995) An examination of the structure of UASB granules. Water Res 29:2449–2454CrossRefGoogle Scholar
  48. Ross WR (1984) The phenomenon of sludge pelletization in the anaerobic treatment of a maize processing waste. Water SA 10:197–204Google Scholar
  49. Rouxhet PG, Mozes N (1990) Physical chemistry of the interaction between attached microorganisms and their support. Water Sci Technol 22:1–16Google Scholar
  50. Schmidt JE, Ahring BK (1994) Extracellular polymers in granular sludge from different upflow anaerobic sludge blanket (UASB) reactors. Appl Microbiol Biotechnol 42:457–462Google Scholar
  51. Schmidt JE, Ahring BK (1996) Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors. Biotechnol Bioeng 49:229–246Google Scholar
  52. Shen CF, Kosaric N, Blaszczyk R (1993) The effect of selected heavy metals (Ni, Co and Fe) on anaerobic granules and their extracellular polymeric substance (EPS). Water Res 27:25–33CrossRefGoogle Scholar
  53. Singh KK, Vincent WS (1987) Clumping characteristics and hydrophobic behavior of an isolated bacterial strain from sewage sludge. Appl Microbiol Biotechnol 25:396–398Google Scholar
  54. Singleton DR, Masuoka J, Hazen KC (2001) Cloning and analysis of a Candida albicans gene that affects cell surface hydrophobicity. J Bacteriol 183:3582–3588CrossRefPubMedGoogle Scholar
  55. Sponza DT (2002) Extracellular polymer substances and physicochemical properties of flocs in steady- and unsteady-state activated sludge systems. Process Biochem 37:983–998CrossRefGoogle Scholar
  56. Strand SP, Varum KM, Ostgaard K (2003) Interactions between chitosans and bacterial suspensions: adsorption and flocculation. Colloids Surf B 27:71–81CrossRefGoogle Scholar
  57. Tay JH, Liu QS, Liu Y (2001a) The role of cellular polysaccharides in the formation and stability of aerobic granules. Lett Appl Microbiol 33:222–226CrossRefPubMedGoogle Scholar
  58. Tay JH, Liu QS, Liu Y (2001b) The effects of shear force on the formation, structure and metabolism of aerobic granules. Appl Microbiol Biotechnol 57:227–233PubMedGoogle Scholar
  59. Tay JH, Liu QS, Liu Y (2001c) Microscopic observation of aerobic granulation in sequential aerobic sludge blanket reactor. J Appl Microbiol 91:168–175CrossRefPubMedGoogle Scholar
  60. Tay JH, Yang SF, Liu Y (2002) Hydraulic selection pressure-induced nitrifying granulation in sequencing batch reactors. Appl Microbiol Biotechnol 59:332–337CrossRefPubMedGoogle Scholar
  61. Trevors JT (1984) The measurement of electron transport system (ETS) activity in freshwater sediment. Water Res 18:581–584CrossRefGoogle Scholar
  62. Tsuneda S, Jung J, Hayashi H, Aikawa H, Hirata A, Sasaki H (2003a) Influence of extracellular polymers on electrokinetic properties of heterotrophic bacterial cells examined by soft particle electrophoresis theory. Colloids Surf B 29:181–188CrossRefGoogle Scholar
  63. Tsuneda S, Aikawa H, Hayashi H, Yuasa A, Hirata A (2003b) Extracellular polymeric substances responsible for bacterial adhesion onto solid surface. FEMS Microbiol Lett 223:287–292CrossRefPubMedGoogle Scholar
  64. Urbain V, Block JC, Manem J (1993) Bioflocculation in activated sludge, an analytic approach. Water Res 27:829–838Google Scholar
  65. Veiga MC, Jain MK, Wu WM, Hollingsworth RI, Zeikus JG (1997) Composition and role of extracellular polymers in methanogenic granules. Appl Environ Microbiol 63:403–407Google Scholar
  66. Yang SF, Tay JH, Liu Y (2003) A novel granular sludge sequencing-batch reactor for organic and nitrogen removal from wastewater. J Biotechnol 106:77–86CrossRefPubMedGoogle Scholar
  67. Yang SF, Tay JH, Liu Y (2004) Inhibition of free ammonia to the formation of aerobic granules. Biochem Eng J 17:41–48CrossRefGoogle Scholar
  68. Yi S, Tay JH, Maszenan AM, Tay STL (2003) A culture-independent approach for studying microbial diversity in aerobic granules. Water Sci Technol 47:283–290Google Scholar
  69. Yu HQ, Tay JH, Fang HHP (2001) The roles of calcium in sludge granulation during UASB reactor start-up. Water Res 35:1052–1060CrossRefPubMedGoogle Scholar
  70. Zhang XQ, Bishop PL (2001) Spatial distribution of extracellular polymeric substances in biofilms. J Environ Eng 127:850–856CrossRefGoogle Scholar
  71. Zhang XQ, Bishop PL, Kinkle BK (1999) Comparison of extraction methods for quantifying extracellular polymers in biofilms. Water Sci Technol 39:211–218CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Division of Environmental and Water Resources Engineering, School of Civil and Environmental EngineeringNanyang Technological UniversitySingapore

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