Applied Microbiology and Biotechnology

, Volume 65, Issue 5, pp 520–529 | Cite as

Improvement of fermentative hydrogen production: various approaches

  • Kaushik Nath
  • Debabrata DasEmail author


Fermentation of biomass or carbohydrate-based substrates presents a promising route of biological hydrogen production compared with photosynthetic or chemical routes. Pure substrates, including glucose, starch and cellulose, as well as different organic waste materials can be used for hydrogen fermentation. Among a large number of microbial species, strict anaerobes and facultative anaerobic chemoheterotrophs, such as clostridia and enteric bacteria, are efficient producers of hydrogen. Despite having a higher evolution rate of hydrogen, the yield of hydrogen [mol H2 (mol substrate−1)] from fermentative processes is lower than that achieved using other methods; thus, the process is not economically viable in its present form. The pathways and experimental evidence cited in the literature reveal that a maximum of four mol of hydrogen can be obtained from substrates such as glucose. Modifications of the fermentation process, by redirection of metabolic pathways, gas sparging and maintaining a low partial pressure of hydrogen to make the reaction thermodynamically favorable, efficient product removal, optimum bioreactor design and integrating fermentative process with that of photosynthesis, are some of the ways that have been attempted to improve hydrogen productivity. This review briefly describes recent advances in these approaches towards improvement of hydrogen yield by fermentation.


Fermentation Hydrogen Production Hydrogen Yield Pressure Swing Adsorption Hydrogen Production Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Adams MWW, Stiefel EI (1998) Biological hydrogen production: not so elementary. Science 282:1842–1843CrossRefPubMedGoogle Scholar
  2. Benemann JR (1996) Hydrogen biotechnology: progress and prospects. Nat Biotechnol 14:1101–1103PubMedGoogle Scholar
  3. Benemann JR (2000) Hydrogen production by microalgae. J Appl Phycol 12:291–300CrossRefGoogle Scholar
  4. Boyles D (1984) Bioenergy technology-Thermodynamics and costs. Wiley, New YorkGoogle Scholar
  5. Chang J-S, Lee K-S, Lin P-J (2002) Biohydrogen production with fixed-bed bioreactors. Int J Hydrogen Energy 27:1167–1174CrossRefGoogle Scholar
  6. Classen PAM, van Lier JB, Lopez Contreras AM, van Niel EWJ, Sijtsma L, Stams AJM, de Vries SS, Weusthuis RA (1999) Utilisation of biomass for the supply of energy carriers. Appl Microbiol Biotechnol 52:741–755CrossRefGoogle Scholar
  7. Classen PAM, van Groenestijn JW, Janssen AJH, van Niel EJW, Wijffels RH (2000) Feasibility of biological hydrogen production from biomass for utilization in fuel cells. In: Proceedings of the 1st World Conference and Exhibition on Biomass for Energy and Industry, Sevilla Spain, 5–9 June 2000Google Scholar
  8. Das D, Veziroglu TN (2001) Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy 26:13–28CrossRefGoogle Scholar
  9. Elam CC, Gregoire Padro CE, Sandrock G, Luzzi A, Lindblad P, Hagen E-F (2003) Realizing the hydrogen future: the International Energy Agency’s efforts to advance hydrogen energy technologies. Int J Hydrogen Energy 28:601–607CrossRefGoogle Scholar
  10. Fabiano B, Perego P (2002) Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes. Int J Hydrogen Energy 27:149–156CrossRefGoogle Scholar
  11. Fang HHP, Liu H (2002) Effect of pH on hydrogen production from glucose by a mixed culture. Bioresour Technol 82:87–93CrossRefPubMedGoogle Scholar
  12. Fascetti E, D’addario E, Todini O, Robertiello A (1998) Photosynthtic hydrogen evolution with volatile organic acids derived from the fermentation of source selected municipal solid wastes. Int J Hydrogen Energy 23:753–760CrossRefGoogle Scholar
  13. Hallenbeck PC, Benemann JR (2002) Biological hydrogen production; fundamentals and limiting processes. Int J Hydrogen Energy 27:1185–1193CrossRefGoogle Scholar
  14. Hawkes FR, Dindale R, Hawkes DL, Hussy I (2002) Sustainable fermentative biohydrogen: challenges for process optimization. Int J Hydrogen Energy 27:1339–1347CrossRefGoogle Scholar
  15. Hussy I, Hawkes FR, Dinsdale R, Hawkes DL (2003) Continuous fermentative hydrogen production from a wheat starch co-product by mixed microflora. Biotechnol Bioeng 84(6):619–626CrossRefPubMedGoogle Scholar
  16. Kataoka N, Miya A, Kiriyama K (1997) Studies on hydrogen production by continuous culture system of hydrogen producing anaerobic bacteria. Water Sci Technol 36:41–47CrossRefGoogle Scholar
  17. Kim MS, Moon KW, Lee SK (2001) Hydrogen production from food processing waste Water and sewage sludge by anaerobic dark fermentation combined with photofermentation. In: Miyake J et al. (eds) Biohydrogen II. Elsevier, Amsterdam, pp 263–272Google Scholar
  18. Koku H, Eroglu I, Gunduz U, Yucel M, Turker L (2002) Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides. Int J Hydrogen Energy 27:1315–1329CrossRefGoogle Scholar
  19. Kondo T, Arakawa M, Waakayama T, Miyake J (2002) Hydrogen production by combining two types of photosynthetic bacteria with different characteristics. Int J Hydrogen Energy 27:1303–1308CrossRefGoogle Scholar
  20. Kumar N, Das D (2000) Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. Process Biochem 35:589–593CrossRefGoogle Scholar
  21. Kumar N, Das D (2001) Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. Enzyme Microbiol Technol 29:280–287CrossRefGoogle Scholar
  22. Kumar N, Ghosh A, Das D (2001) Redirection of biochemical pathways for the enhancement of H2 production by Enterobacter cloacae. Biotechnol Lett 23:537–541CrossRefGoogle Scholar
  23. Lay J-J (2000) Modelling and optimization of anaerobic digested sludge converting starch to hydrogen. Biotechnol Bioeng 68(3):269–278CrossRefPubMedGoogle Scholar
  24. Lay J-J, Lee Y-J, Noike T (1999) Feasibility of biological hydrogen production from organic fraction of municipal solid waste. Water Res 33(11):2579–2586CrossRefGoogle Scholar
  25. Lee C-M, Chen P-C, Wang C-C, Tung Y-C (2002) Photohydrogen production using purple non-sulfur bacteria with hydrogen fermentation reactor effluent. Int J Hydrogen Energy 27:1308–1314Google Scholar
  26. Levin DB, Pitt L, Love M (2004) Biohydrogen production: prospects and limitations to practical application. Int J Hydrogen Energy 29: 173–185CrossRefGoogle Scholar
  27. Liang T-M, Cheng S-S, Wu K-L (2002) Behavioral study on hydrogen fermentation reactor installed with silicone rubber membrane. Int J Hydrogen Energy 27:1157–1165CrossRefGoogle Scholar
  28. Lin C-Y, Chang R-C (1999) Hydrogen production during anaerobic acidogenic conversion of glucose. J Chem Technol Biotechnol 74(6):496–500CrossRefGoogle Scholar
  29. Lindblad P, Christensson K, Lindberg P, Fedorov A, Pinto F, Tsygankov A (2002) Photoproduction of H2 by wild type AnabaenaPCC 1720 and a hydrogen uptake deficient mutant: from laboratory to outdoor culture. Int J Hydrogen Energy 27:1271–1281CrossRefGoogle Scholar
  30. Mahyudin AR, Furutani Y, Nakashimada Y, Kakizono T, Nishio (1997) Enhanced hydrogen production in altered mixed acid fermentation of glucose by Enterobacter aerogenes. J Ferm Bioeng 83(4):358–363CrossRefGoogle Scholar
  31. Mizuno O, Dinsdale R, Hawkes FR, Hawkes DL, Noike T (2000) Enhancement of hydrogen production from glucose by nitrogen gas sparging. Bioresour Technol 73:59–65CrossRefGoogle Scholar
  32. Nandi R, Sengupta S (1998) Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84PubMedGoogle Scholar
  33. Nielsen AT, Amandusson H, Bjorklund R, Dannetun H, Ejlertsson J, Ekedahl L-G, Lundstrom I, Svensson B (2001) Hydrogen production from organic waste. Int J Hydrogen Energy 26:547–550CrossRefGoogle Scholar
  34. Oh Y-K, Seol E-H, Yeol Lee E, Park S (2002) Fermentative hydrogen production by a new chemolithotrophic bacterium Rhodopseudomonas palustris P4. Int J Hydrogen Energy 27:1373–1379CrossRefGoogle Scholar
  35. Oh Y-K, Seol E-H, Kim JR, Park S (2003) Fermentative biohydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19. Int J Hydrogen Energy 28:1353–1359CrossRefGoogle Scholar
  36. Rao KK, Hall DO (1996) Hydrogen production by cyanobacteria: potential, problems and prospects. J Mar Biotechnol 4:10–15Google Scholar
  37. Reith JH, Wijffels RH, Barten H (2003) Biomethane and biohydrogen-status and perspective of biological methane and hydrogen production. Dutch biological hydrogen foundation, The Netherelands, pp 118–125Google Scholar
  38. Tanisho S (2000) A strategy for improving the yield of hydrogen by fermentation. In: Mao ZQ, Veziroglu TN (eds) Hydrogen Energy Progress XIII, vol 1, Beijing, China, pp 370–374Google Scholar
  39. Tanisho S, Kuromoto M, Kadokura N (1998) Effect of CO2 removal on hydrogen production by fermentation. Int J Hydrogen Energy 23:559–563CrossRefGoogle Scholar
  40. Teplyakov VV, Gassanova LG, Sostina EG, Slepova EV, Modigell M, Netrusov AI (2002) Lab scale bioreactor integration with active membrane system for hydrogen production: experience and prospects. Int J Hydrogen Energy 27:1149–1155CrossRefGoogle Scholar
  41. Tredici MR, Chini Zittelli G, Benemann JR (1998) A tubular integral gas exchange photobioreactor for biological hydrogen production. In: Zaborsky OR (ed) Biohydrogen. Plenum, London, pp 391–401Google Scholar
  42. Van Groenestijn JW, Hazewinkel JHO, Nienoord M, Bussmann PJT (2002) Energy aspects of biological hydrogen production in high rate bioreactors operated in the thermophilic temperature range. Int J Hydrogen Energy 27:1141–1147CrossRefGoogle Scholar
  43. Van Niel EWJ, Claassen PAM, Starns AJM (2003) Substrate and product inhibition of hydrogen production by the extreme thermophile Caldicellulosiruptor saccharolyticus. Biotechnol Bioeng 81:255–262CrossRefPubMedGoogle Scholar
  44. Woodward J, Mattingly SM, Danson M, Hough D, Ward N, Adams M (1996) In vitro hydrogen production by glucose dehydrogenase and hydrogenase. Nat Biotechnol 14:872–874PubMedGoogle Scholar
  45. Woodward J, Orr M, Cordray K, Greenbaum E (2000) Enzymatic production of biohydrogen. Nature 405:1014–1015CrossRefPubMedGoogle Scholar
  46. Yokoi H, Mori S, Hirose J, Hayashi S, Takasaki Y (1998) H2 production from starch by a mixed culture of Clostridium butyricum and Rhodobacter sp M-19. Biotechnol Lett 20:895–890CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of BiotechnologyIndian Institute of TechnologyKharagpurIndia

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