Applied Microbiology and Biotechnology

, Volume 65, Issue 5, pp 576–582 | Cite as

Identification and characterization of the main β-alanine uptake system in Escherichia coli

  • Frank Schneider
  • Reinhard Krämer
  • Andreas Burkovski
Applied Genetics and Molecular Biotechnology

Abstract

In Escherichia coli, β-alanine is a direct precursor in the biosynthesis of pantothenic acid (vitamin B5). Although a sufficient β-alanine supply is crucial for biotechnological vitamin B5 production, nothing was known about β-alanine transport in E. coli until now. The aim of this work was the characterization of β-alanine transport by E. coli and the identification and overexpression of the corresponding carrier-encoding gene for the rational improvement of pantothenic acid-producing strains. β-Alanine uptake was found to be an active process catalyzed by the amino acid carrier CycA. The corresponding gene was cloned and overexpressed, resulting in an increase in the uptake rate, compared with the wild type. In all tested strains, this overexpression led to a strong sensitivity to β-alanine, but not to the other CycA substrates, such as l-alanine, d-alanine, and glycine. This prevented a direct application for the improvement of pantothenic acid-producing strains by an enhanced precursor supply.

Keywords

Carnosine Pantothenic Acid Pantothenate Biosynthesis Ketopantoate Amino Acid Transporter Family 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This work was supported by Degussa AG (Hanau).

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Frank Schneider
    • 1
  • Reinhard Krämer
    • 1
  • Andreas Burkovski
    • 1
  1. 1.Institut für BiochemieUniversität KölnCologneGermany

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