Applied Microbiology and Biotechnology

, Volume 65, Issue 3, pp 259–262

Production of antithrombotic hirudin in GAL1-disrupted Saccharomyces cerevisiae

Biotechnological Products and Process Engineering


A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose supply on hirudin expression in the gratuitous strain. Controlled feeding of glucose successfully supported both cell growth and hirudin expression in the gratuitous strain. The optimum fed-batch culture done by feeding glucose at a rate of 0.3 g h−1 produced a maximum hirudin concentration of 62.1 mg l−1, which corresponded to a 4.5-fold increase when compared with a simple batch culture done with the same strain.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of Genetics and Development, and MicrobiologyColumbia UniversityNew YorkUSA
  2. 2.Department of Agricultural Biotechnology and Research Center for New Bio-Materials in AgricultureSeoul National UniversitySeoulSouth Korea
  3. 3.Mogam Biotechnology InstituteYonginSouth Korea

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