Applied Microbiology and Biotechnology

, Volume 64, Issue 5, pp 625–635 | Cite as

Secretory and extracellular production of recombinant proteins using Escherichia coli

  • J. H. Choi
  • S. Y. LeeEmail author


Escherichia coli is one of the most widely used hosts for the production of recombinant proteins. However, there are often problems in recovering substantial yields of correctly folded proteins. One approach to solve these problems is to have recombinant proteins secreted into the periplasmic space or culture medium. The secretory production of recombinant proteins has several advantages, such as simplicity of purification, avoidance of protease attack and N-terminal Met extension, and a better chance of correct protein folding. In addition to the well-established Sec system, the twin-arginine translocation (TAT) system has recently been employed for the efficient secretion of folded proteins. Various strategies for the extracellular production of recombinant proteins have also been developed. For the secretory production of complex proteins, periplasmic chaperones and protease can be manipulated to improve the yields of secreted proteins. This review discusses recent advances in secretory and extracellular production of recombinant proteins using E. coli.


Recombinant Protein Disulfide Bond Secretory Production TMAO Periplasmic Space 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This review was supported by the Korean Systems Biology Research Grant (M10309020000-03B5002-00000) from the Ministry of Science and Technology. Support from IBM through the IBM-SUR program is greatly appreciated.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Metabolic and Biomolecular Engineering National Research LaboratoryDepartment of Chemical and Biomolecular Engineering and BioProcess Engineering Research CenterYuseong-guKorea
  2. 2.Department of Biosystems and Bioinformatics Research CenterKorea Advanced Institute of Science and TechnologyYuseong-guKorea

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