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Applied Microbiology and Biotechnology

, Volume 64, Issue 6, pp 848–854 | Cite as

Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta)

  • J. A. Del Campo
  • H. Rodríguez
  • J. Moreno
  • M. Á. Vargas
  • J. Rivas
  • M. G. GuerreroEmail author
Original Paper

Abstract

When grown photoautotrophically, Chlorella zofingiensis strain CCAP 211/14 accumulates a significant amount of valuable carotenoids, namely astaxanthin and lutein, of increasing demand for use as feed additives in fish and poultry farming, as colorants in food, and in health care products. Under standard batch-culture conditions, this microalgal strain exhibits high values of both growth rate (about 0.04 h−1) and standing cell population (over 1011 cells l−1, or 7 g dry weight l−1). Lutein, in a free (unesterified) form, was the prevalent carotenoid during early stages of cultivation (over 0.3 pg cell−1, equal to 4 mg g−1 dry weight, or 20 mg l−1 culture), whereas esterified astaxanthin accumulated progressively, to reach a maximum (over 0.1 pg cell−1, equal to 1.5 mg g−1 dry weight, or 15 mg l−1 culture) in the late stationary phase. A differential response of lutein and astaxanthin accumulation was also recorded with regard to the action of some environmental and nutritional factors. C. zofingiensis CCAP 211/14 represents a unique model system for analyzing the differential regulation of the levels of primary (lutein) and secondary (astaxanthin) carotenoids. Relevant also from the biotechnological viewpoint, this photosynthetic organism, with outstanding attributes for fast photosynthetic growth and carotenoid accumulation, might prove most valuable for its application to the mass production of either or both lutein and astaxanthin.

Keywords

Carotenoid Lutein Astaxanthin Total Carotenoid Canthaxanthin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The valuable technical assistance of M.J. Figueroa and A.M. Blanco is very much appreciated. This work was supported by Plan Nacional, Ministerio de Ciencia y Tecnología (grants BIO97-0577, PPQ2001-3832-C02-01, cofinanced with FEDER funds from the European Union) and Plan Andaluz de Investigación (group no. CVI 0131), Spain.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • J. A. Del Campo
    • 1
  • H. Rodríguez
    • 1
  • J. Moreno
    • 1
  • M. Á. Vargas
    • 1
  • J. Rivas
    • 1
  • M. G. Guerrero
    • 1
    Email author
  1. 1.Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la CartujaConsejo Superior de Investigaciones Científicas–Universidad de SevillaSevillaSpain

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