Applied Microbiology and Biotechnology

, Volume 64, Issue 3, pp 376–381

Non-conventional yeasts as producers of polyhydroxyalkanoates—genetic engineering of Arxula adeninivorans

Original Paper

DOI: 10.1007/s00253-003-1498-x

Cite this article as:
Terentiev, Y., Breuer, U., Babel, W. et al. Appl Microbiol Biotechnol (2004) 64: 376. doi:10.1007/s00253-003-1498-x

Abstract

The non-conventional yeast Arxula adeninivorans was equipped with the genes phbA, phbB and phbC of the polyhydroxyalkanoate (PHA) biosynthetic pathway of Ralstonia eutropha, which encode β-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. Arxula strains transformed solely with the PHA synthase gene (phbC) were able to produce PHA. However, the maximum content of the polymer detected in these strains was just 0.003% poly-3-hydroxybutyrate (PHB) and 0.112% poly-3-hydroxyvalerate (PHV). The expression of all three genes (phbA, phbB, phbC) resulted in small increases in the PHA content of the transgenic Arxula cells. However, under controlled cultivation conditions with minimal medium and ethanol as the carbon source, the recombinant yeast was able to accumulate up to 2.2% PHV and 0.019% PHB. Possible reasons for these differences are discussed.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany
  2. 2.Center for Environmental Research Leipzig-Halle (UFZ)LeipzigGermany

Personalised recommendations