Applied Microbiology and Biotechnology

, Volume 63, Issue 6, pp 748–753 | Cite as

The effect of CreA in glucose and xylose catabolism in Aspergillus nidulans

Original Paper


The catabolism of glucose and xylose was studied in a wild type and creA deleted (carbon catabolite de-repressed) strain of Aspergillus nidulans. Both strains were cultivated in bioreactors with either glucose or xylose as the sole carbon source, or in the presence of both sugars. In the cultivations on single carbon sources, it was demonstrated that xylose acted as a carbon catabolite repressor (xylose cultivations), while the enzymes in the xylose utilisation pathway were also subject to repression in the presence of glucose (glucose cultivations). In the wild type strain growing on the sugar mixture, glucose repression of xylose utilisation was observed; with xylose utilisation occurring only after glucose was depleted. This phenomenon was not seen in the creA deleted strain, where glucose and xylose were catabolised simultaneously. Measurement of key metabolites and the activities of key enzymes in the xylose utilisation pathway revealed that xylose metabolism was occurring in the creA deleted strain, even at high glucose concentrations. Conversely, in the wild type strain, activities of the key enzymes for xylose metabolism increased only when the effects of glucose repression had been relieved. Xylose was both a repressor and an inducer of xylanases at the same time. The creA mutation seemed to have pleiotropic effects on carbohydratases and carbon catabolism.



This work was carried out in the framework of the QTRK3-1999-00729 programme, within the Eurofung consortium, financially supported by the EU.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Centre for Process Biotechnology, BioCentrum-DTU, Building 223Technical University of DenmarkLyngbyDenmark

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