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Applied Microbiology and Biotechnology

, Volume 63, Issue 3, pp 293–299 | Cite as

Overexpression of the ATP-dependent helicase RecG improves resistance to weak organic acids in Escherichia coli

  • P. Steiner
  • U. Sauer
Original Paper

Abstract

Increased resistance to several weak organic acids was conferred on Escherichia coli by overexpression of the ATP-dependent helicase RecG and, to a lesser extent, by overexpressing the helicase RuvAB. This property of helicases was identified by reproducible selection of recG-bearing clones from genomic libraries of the acetate-resistant species Acetobacter aceti and Staphylococcus capitis. We show that overexpression of RecG from both species, but also from E. coli, increased the maximum biomass concentration attained by E. coli cultures that were grown in the presence of various weak organic acids and uncouplers. Furthermore, overexpression of RecG from A. aceti significantly improved the maximum growth rates of E. coli under weak organic acid challenge. Based on the known role of RecG in DNA replication/repair, our data provide a first indication that weak organic acids negatively affect DNA replication and/or repair, and that these negative effects may be counteracted by helicase activity.

Keywords

Genomic Library Luria Broth Maximum Specific Growth Rate Restriction Enzyme Analysis Acetic Acid Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank E. Marti for constructing the S. capitis library and for help with the selection experiment, H. Ernst for sequencing, and R. Stephan for providing chromosomal DNA of E. coli O157:H7. Funding from the ETH Forschungskommision is acknowledged.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Institute of BiotechnologyETH ZürichZürichSwitzerland

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