Applied Microbiology and Biotechnology

, Volume 64, Issue 2, pp 275–283 | Cite as

Inhibiting mild steel corrosion from sulfate-reducing bacteria using antimicrobial-producing biofilms in Three-Mile-Island process water

  • R. Zuo
  • D. Örnek
  • B. C. Syrett
  • R. M. Green
  • C.-H. Hsu
  • F. B. Mansfeld
  • T. K. Wood
Original Paper

Abstract

Biofilms were used to produce gramicidin S (a cyclic decapeptide) to inhibit corrosion-causing, sulfate-reducing bacteria (SRB). In laboratory studies these biofilms protected mild steel 1010 continuously from corrosion in the aggressive, cooling service water of the AmerGen Three-Mile-Island (TMI) nuclear plant, which was augmented with reference SRB. The growth of both reference SRB (Gram-positive Desulfosporosinus orientis and Gram-negative Desulfovibrio vulgaris) was shown to be inhibited by supernatants of the gramicidin-S-producing bacteria as well as by purified gramicidin S. Electrochemical impedance spectroscopy and mass loss measurements showed that the protective biofilms decreased the corrosion rate of mild steel by 2- to 10-fold when challenged with the natural SRB of the TMI process water supplemented with D. orientis or D. vulgaris. The relative corrosion inhibition efficiency was 50–90% in continuous reactors, compared to a biofilm control which did not produce the antimicrobial gramicidin S. Scanning electron microscope and reactor images also revealed that SRB attack was thwarted by protective biofilms that secrete gramicidin S. A consortium of beneficial bacteria (GGPST consortium, producing gramicidin S and other antimicrobials) also protected the mild steel.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • R. Zuo
    • 1
  • D. Örnek
    • 1
    • 5
  • B. C. Syrett
    • 2
  • R. M. Green
    • 3
  • C.-H. Hsu
    • 4
  • F. B. Mansfeld
    • 4
  • T. K. Wood
    • 1
  1. 1.Departments of Chemical Engineering & Molecular and Cell BiologyUniversity of ConnecticutStorrsUSA
  2. 2.Electric Power Research InstitutePalo AltoUSA
  3. 3.TMI Nuclear Generation StationMiddletownUSA
  4. 4.Department of Materials Science & EngineeringUniversity of Southern CaliforniaLos AngelesUSA
  5. 5.Genzyme CorporationCambridgeUSA

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