Applied Microbiology and Biotechnology

, Volume 63, Issue 5, pp 549–552 | Cite as

Consortium of fold-catalyzing proteins increases soluble expression of cyclohexanone monooxygenase in recombinant Escherichia coli

  • D.-H. Lee
  • M.-D. Kim
  • W.-H. Lee
  • D.-H. Kweon
  • J.-H. SeoEmail author
Original Paper


The cyclohexanone monooxygenase (CHMO) gene of Acinetobacter sp. NCIMB 9871 was simultaneously expressed with the genes encoding molecular chaperones and foldases in Escherichia coli. While the expression of the CHMO gene alone resulted in the formation of inclusion bodies, coexpression of the chaperone or foldase genes remarkably increased the production of soluble CHMO enzyme in recombinant E. coli. Furthermore, it was found that molecular chaperones were more beneficial than foldases for enhancing active CHMO enzyme production. The recombinant E. coli strain simultaneously expressing the genes for CHMO, GroEL/GroES and DnaK/DnaJ/GrpE showed a specific CHMO activity of 111 units g−1 cell protein, corresponding to a 38-fold enhancement in CHMO activity compared with the control E. coli strain expressing the CHMO gene alone.


Molecular Chaperone Coli Strain Soluble Expression Folding Pathway Villiger Oxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Professor Jon D. Stewart (University of Florida, Gainesville, Fla.) for his kind donation of the plasmid pMM4. This work was supported by the Center for Advanced Bioseparation Technology and the Ministry of Education, through the BK21 program.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • D.-H. Lee
    • 1
  • M.-D. Kim
    • 1
  • W.-H. Lee
    • 1
  • D.-H. Kweon
    • 2
  • J.-H. Seo
    • 1
    Email author
  1. 1.Department of Agricultural BiotechnologySeoul National UniversitySuwonSouth Korea
  2. 2.Food Processing Division, School of Bioresource SciencesAndong National UniversityAndongSouth Korea

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