Applied Microbiology and Biotechnology

, Volume 62, Issue 4, pp 331–336 | Cite as

Cleavage of β,β-carotene to flavor compounds by fungi

  • H. Zorn
  • S. Langhoff
  • M. Scheibner
  • R. G. Berger
Original Paper


More than 50 filamentous fungi and yeasts, known for de novo synthesis or biotransformation of mono-, sesqui-, tri-, or tetraterpenes, were screened for their ability to cleave β,β-carotene to flavor compounds. Ten strains discolored a β,β-carotene-containing growth agar, indicating efficient degradation of β,β-carotene. Dihydroactinidiolide was formed as the sole conversion product of β,β-carotene in submerged cultures of Ganoderma applanatum, Hypomyces odoratus, Kuehneromyces mutabilis, and Trametes suaveolens. When mycelium-free culture supernatants from five species were applied for the conversions, nearly complete degradation of β,β-carotene was observed after 12 h. Carotenoid-derived volatile products were detected in the media of Ischnoderma benzoinum, Marasmius scorodonius, and Trametes versicolor. β-Ionone proved to be the main metabolite in each case, whereas β-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed in minor quantities. Using a photometric bleaching test, the β,β-carotene cleaving enzyme activities of M. scorodonius were partially characterized.


Carotenoid Submerged Culture Extracellular Enzyme Activity Carotenoid Degradation Ganoderma Applanatum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Support of the work by the Deutsche Forschungsgemeinschaft (ZO 122/1–1) is gratefully acknowledged. All experiments comply with the current laws of the Federal Republic of Germany.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • H. Zorn
    • 1
  • S. Langhoff
    • 1
  • M. Scheibner
    • 1
  • R. G. Berger
    • 1
  1. 1.Zentrum Angewandte ChemieInstitut für Lebensmittelchemie der Universität HannoverHannoverGermany

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