Applied Microbiology and Biotechnology

, Volume 62, Issue 2–3, pp 284–290 | Cite as

A novel catabolic activity of Pseudomonas veronii in biotransformation of pentachlorophenol

  • I.-H. Nam
  • Y.-S. ChangEmail author
  • H.-B. Hong
  • Y.-E. Lee
Original Paper


Pseudomonas veronii PH-05, a bacterial strain capable of transforming pentachlorophenol (PCP) to a metabolic intermediate, was isolated by selective enrichment of soil samples from a timber storage yard. Strain PH-05 was shown to be able to grow using PCP as the sole source of carbon and energy. GC-MS analysis showed that the metabolic intermediate was tetrachlorocatechol, which inhibited the growth of this strain. The formation of tetrachlorocatechol during biotransformation was monitored, and its inhibitory effect on growth of strain PH-05 was analyzed at a range of concentrations. The catabolic activity of the isolated strain differs from that of other PCP-degrading bacteria, which metabolize PCP through a chlorinated hydroquinone intermediate.


Metabolic Intermediate Dichlorophenol Minimal Salt Medium Chlorocatechol Iodomethane 
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This work was financially supported by Korea Research Foundation grant (KRF-2001-015-DP 0440).


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.School of Environmental Science and EngineeringPohang University of Science and TechnologyHyojadong 790–784Korea
  2. 2.Department of BiochemistryDongguk UniversityKyungju 780–714Korea

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