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Applied Microbiology and Biotechnology

, Volume 61, Issue 3, pp 261–267 | Cite as

Phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning bioreactors

  • R. Muñoz
  • B. Guieysse
  • B. MattiassonEmail author
Original Paper

Abstract

An algal-bacterial consortium formed by Chlorella sorokiniana and a phenanthrene-degrading Pseudomonas migulae strain was able to biodegrade 200–500 mg/l of phenanthrene dissolved in silicone oil or tetradecane under photosynthetic conditions and without any external supply of oxygen. Phenanthrene was only removed when provided in organic solvent, which confirms the potential of two-phase systems for toxicity reduction. Phenanthrene was degraded at highest rates when provided in silicone oil rather than in tetradecane since this solvent probably sequestered the PAH, reducing its mass transfer to the aqueous phase. The influence of phenanthrene concentration, amount of inoculum and light intensity on pollutant removal was also investigated and, under the best conditions, phenanthrene was degraded at 24.2 g m−3·h−1. In addition to being cost-effective and mitigating the release of greenhouse gases into the atmosphere, photosynthetic oxygenation was especially beneficial to the use of two-phase partitioning bioreactors since it prevented solvent emulsification and/or volatilization and evidence was found that the microalgae release biosurfactants that could further enhance phenanthrene degradation.

Keywords

Phenanthrene Chlorella Biosurfactants Mineral Salt Medium Biosurfactant Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Raul Muñoz and Benoit Guieysse worked within the COLDREM project (soil Remediation in a Cold Climate) and were supported by Mistra (Foundation for Strategic Environmental Research).

References

  1. Bell J, Melcer H, Monteith H, Osinga I, Steel P (1993) Stripping of volatile organic compounds at full-scale municipal wastewater treatment plants. Water Environ Res 65:708–716Google Scholar
  2. Borde X, Guieysse B, Delgado O, Hatti-Kaul R, Nugier-Chauvin C, Patin H, Mattiasson B (2002). Synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants. Bioresour Technol 86:293–300CrossRefGoogle Scholar
  3. Bouchez M, Blanchet D, Vandecasteele JP (1995) Substrate availability in phenanthrene biodegradation: transfer mechanism and influence on metabolism. Appl Microbiol Biotechnol 43:952–960PubMedGoogle Scholar
  4. Bruce LJ, Daugulis AJ (1991) Solvent selection strategies for extractive biocatalysis. Biotechnol Prog 7:116–124PubMedGoogle Scholar
  5. Cerniglia CE (1992) Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3:351–368Google Scholar
  6. Cirigliano M, Carman G (1985) Purification and characterization of Liposan, a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50:847–850Google Scholar
  7. Collins LD, Daugulis AJ (1997) Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors. Biotechnol Bioeng 55:155–162Google Scholar
  8. Daugulis AJ (1997) Partitioning bioreactors. Curr Opin Biotechnol 8:169–174Google Scholar
  9. Daugulis AJ (2001) Two-phase partitioning bioreactors: a new technology platform for destroying xenobiotics. Trends Biotechnol 19:457–462CrossRefPubMedGoogle Scholar
  10. Daugulis AJ, Janikowski TB (2002) Scale-up performance of a partitioning bioreactor for the degradation of polyaromatic hydrocarbons by Sphingomonas aromaticivorans. Biotechnol Lett 24:591–594.CrossRefGoogle Scholar
  11. Déziel E, Comeau Y, Villemur R (1999) Two liquid-phase bioreactors for enhanced degradation of hydrophobic/toxic compounds. Biodegradation 10:219–233CrossRefPubMedGoogle Scholar
  12. Doddamani HP, Ninnekar HZ (2000) Biodegradation of phenanthrene by a Bacillus species. Curr Microbiol 41:11–14PubMedGoogle Scholar
  13. Efroymson RA, Alexander M (1991) Biodegradation by an Arthrobacter species of hydrocarbons partitioned into an organic solvent. Appl Environ Microbiol 57:1441–1447Google Scholar
  14. El Aalam S, Pauss A, Lebeault JM (1993) High efficiency styrene biodegradation in a biphasic organic/water continuous reactor. Appl Microbiol Biotechnol 39:696–699Google Scholar
  15. Guieysse B, Cirne MDTG, Mattiasson B (2001a) Microbial degradation of phenanthrene and pyrene in a two-liquid phase partitioning bioreactor. Appl Microbiol Biotechnol 56:796–802CrossRefPubMedGoogle Scholar
  16. Guieysse B, Lundstedt S, van Bavel B, Mattiasson B (2001b) Biological treatment of PAH contaminated soil extracts. In: Magar VS, Johnson G, Ong SK, Leeson A (eds) Bioremediation of energetics, phenolics, and polycyclic aromatic hydrocarbons: the sixth international in-situ and on-site bioremediation symposium, 4–7 June 2001, San Diego, Calif. Battelle Press, Columbus Ohio, pp 181–188Google Scholar
  17. Guieysse B, Borde X, Muñoz R, Hatti-Kaul R, Nugier-Chauvin C, Patin H, Mattiasson B (2002) Influence of the initial composition of algal-bacterial microcosms on the degradation of salicylate in a fed-batch culture. Biotechnol Lett 24:531–538CrossRefGoogle Scholar
  18. Janikowski TB, Veligonga D, Punt M, Daugulis AJ (2002) Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp. Appl Microbiol Biotechnol 59:368–376CrossRefPubMedGoogle Scholar
  19. Köhler A, Schüttoff M, Bryniok D, Knackmub HJ (1994) Enhanced biodegradation of phenanthrene in a biphasic culture system. Biodegradation 5:93–103Google Scholar
  20. Mara DD, Pearson HW (1986) Artificial freshwater environment: waste stabilization ponds. In: Rhem HJ, Reed G (eds) Biotechnology, vol 8. VCH, Weinheim, pp 177–206Google Scholar
  21. Metting FR Jr (1996) Biodiversity and application of microalgae. J Ind Microbiol 17:477–489Google Scholar
  22. Nadalig T, Raymond N, Ni'matuzahorh, Gilewicz M, Budzinski H, Bertrand JC (2002) Degradation of phenanthrene, methylphenanthrenes and dibenzothiophene by a Sphingomonas strain 2mpll. Appl Microbiol Biotechnol 59:79–85CrossRefPubMedGoogle Scholar
  23. Ogbonna JC, Tanaka H (2000) Light requirement and photosynthetic cell cultivation—development of processes for efficient light utilization in photobioreactors. J Appl Phycol 12:207–218CrossRefGoogle Scholar
  24. Osswald P, Baveye P, Block JC (1996) Bacterial influence on partitioning rate during the biodegradation of styrene in a biphasic aqueous-organic system. Biodegradation 7:297–302PubMedGoogle Scholar
  25. Oswald JW (1988) Phototrophic microalgae and waste-water treatment. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp 305–328Google Scholar
  26. Richmond A (1983) Phototrophic microalgae. In: Rhem HJ, G Reed (eds) Biotechnology, vol 3. VCH, Weinheim, pp 109–114Google Scholar
  27. Shuttleworth KL, Cerniglia CE (1995) Environmental aspects of PAH biodegradation. Appl Biochem Biotechnol 54:291–302PubMedGoogle Scholar
  28. Sorokin C, Krauss RW (1958) The effect of light intensity on the growth rate of green algae. Plant Physiol 33:1315–1320Google Scholar
  29. Tian L, Ma P, Zhong JJ (2002) Kinetics and key enzyme activities of phenanthrene degradation by Pseudomonas mendocina. Process Biochem 37:1431–1437CrossRefGoogle Scholar
  30. Tschiersch H, Ohmann E (1993) Photoinhibition in Euglena gracilis: involvement of reactive oxygen species. Planta 191:316–323Google Scholar
  31. Villemur R, Déziel E, Benachenhou A, Marcoux J, Gauthier E, Lépine F, Beaudet R, Comeau Y (2000) Two-liquid-phase slurry bioreactors to enhance the degradation of high-molecular-weight polycyclic aromatic hydrocarbons in soil. Biotechnol Prog 16:966–972CrossRefPubMedGoogle Scholar
  32. Vonshak A, Chanawongse L, Bunnag B, Tanticharoen M (1996) Light acclimation in three Spirulina platensis (cyanobacteria) isolates. J Appl Phycol 8:35–40Google Scholar
  33. Yuan SY, Wei SH, Chang BV (2000) Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere 41:1463–1468PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Department of BiotechnologyLund UniversityLundSweden

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