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Applied Microbiology and Biotechnology

, Volume 60, Issue 1–2, pp 60–66 | Cite as

Study of two-stage processes for the microbial production of 1,3-propanediol from glucose

  •  M. Hartlep
  •  W. Hussmann
  •  N. Prayitno
  •  I. Meynial-Salles
  •  A.-P. Zeng
Original Paper

Abstract.

The microbial production of 1,3-propanediol (1,3-PD) from glucose was studied in a two-stage fermentation process on a laboratory scale. In the first stage, glucose was converted to glycerol either by the osmotolerant yeast Pichia farinosa or by a recombinant Escherichia coli strain. In the second stage, glycerol in the broth from the first stage was converted to 1,3-PD by Klebsiella pneumoniae. The culture broth from P. farinosa was shown to contain toxic metabolites that strongly impair the growth of K. pneumoniae and the formation of 1,3-PD. Recombinant E. coli is more suitable than P. farinosa for producing glycerol in the first stage. The fermentation pattern from glycerol can be significantly altered by the presence of acetate, leading to a significant reduction of PD yield in the second stage. However, in the recombinant E. coli culture acetate formation can be prevented by fed-batch cultivation under limiting glucose supply, resulting in an effective production of 1,3-PD in the second stage with a productivity of 2.0 g l–1 h–1 and a high yield (0.53 g/g) close to that of glycerol fermentation in a synthetic medium. The overall 1,3-PD yield from glucose in the two stage-process with E. coli and K. pneumoniae reached 0.17 g/g.

Keywords

Fermentation Glycerol Pichia Klebsiella Klebsiella Pneumoniae 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  M. Hartlep
    • 1
  •  W. Hussmann
    • 1
  •  N. Prayitno
    • 1
  •  I. Meynial-Salles
    • 2
  •  A.-P. Zeng
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Mascheroder Weg 1, 38124 Braunschweig, Germany
  2. 2.CRITT-Bioindustries, CRT, INSA, DGBA, 135 Avenue de Rangueil, 31077 Toulouse cedex4, France

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