Applied Microbiology and Biotechnology

, Volume 59, Issue 6, pp 685–694 | Cite as

Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors

  • G. MikschEmail author
  • S. Kleist
  • K. Friehs
  • E. Flaschel
Original Paper


The gene for phytase from Escherichia coli was sequenced and compared with the appA gene. It was found to be a mutant derivative of the appA gene. After fusion with a C-terminal His-tag, phytase was purified by affinity chromatography and the enzymatic properties were analyzed. To develop a system for overexpression and extracellular production of phytase in E. coli, factors affecting the expression and secretion such as promoter type, host strain and selection pressure were analyzed. Using a secretion system based on the controlled expression of the kil gene, the expression of phytase was improved and the enzyme was released into the culture medium at a high level. An effective fermentation strategy based on fed-batch operation was developed.


Phytic Acid Phytase Activity Extracellular Production Phytase Production Phytase Gene 
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Copyright information

© Springer-Verlag  2003

Authors and Affiliations

  1. 1.Department of Fermentation Engineering, Faculty of Technology, University of Bielefeld, 33501 Bielefeld, GermanyGermany

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