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Adaptation of the white-rot basidiomycete Panus tigrinus for transformation of high concentrations of chlorophenols

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Abstract.

During feed-batch cultivation of the white-rot fungus Panus tigrinus in a 5-l bioreactor on N-limited medium, 100, 200, 500, 1,000 and 2,000 mg 2,4,6-trichlorophenol (2,4,6-TCP) l–1 were added sequentially after 90% removal of the previous portion of the toxicant. The addition of 500 mg 2,4,6-TCP l–1 without preliminary adaptation killed the culture. The addition of 300 mg 2,4,6-TCP l–1 without prior adaptation resulted in its slower removal than removal of 2,000 mg 2,4,6-TCP l–1 by this adapted culture. After adaptation of P. tigrinus to 2,4,6-TCP in a 72-l bioreactor, the mixture of 2,4-dichlorophenol, 2,4,6-TCP, and pentachlorophenol, each at 500 mg l–1, was totally removed over 3 weeks. No lignin peroxidase activity was found in the course of cultivation of the fungus. Laccase activity was suppressed by addition of 2,4,6-TCP. Mn-peroxidase was found to be responsible for transformation of the chlorophenols. As final products of the process, several newly formed aromatic polymers, both chlorinated and non-chlorinated, were found in the culture liquid.

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Leontievsky, .A., Myasoedova, .N., Golovleva, .L. et al. Adaptation of the white-rot basidiomycete Panus tigrinus for transformation of high concentrations of chlorophenols. Appl Microbiol Biotechnol 59, 599–604 (2002). https://doi.org/10.1007/s00253-002-1037-1

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Keywords

  • Lignin
  • Chlorinate
  • Laccase Activity
  • Chlorophenols
  • Preliminary Adaptation