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Applied Microbiology and Biotechnology

, Volume 59, Issue 4–5, pp 529–534 | Cite as

Construction of a stable microbial community with high cellulose-degradation ability

  •  S. Haruta
  •  Z. Cui
  •  Z. Huang
  •  M. Li
  •  M. Ishii
  •  Y. Igarashi
Original Paper

Abstract.

We bred a microbial community capable of degrading rice straw with high efficiency. The microbial community degraded more than 60% of rice straw within 4 days at 50 °C. The high stability of the community's degradation ability was demonstrated by its tolerance of being subcultured several times in medium with/without cellulosic material, being heated to 95 °C, and freezing at –80 °C. The community degraded both nonsterilized and sterilized substrate; and its degradation ability was not affected by pH changes in the medium (initial pH 5–9). PCR-denaturing gradient gel electrophoresis (DGGE) analyses based on 16S rDNA fragments showed that the community structure remained constant after multiple subcultures extending over 2 years. DNA sequence analyses of DGGE bands indicated the coexistence of both aerobic and anaerobic bacteria in the community.

Keywords

Electrophoresis Microbial Community Community Structure Straw Rice Straw 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  S. Haruta
    • 1
  •  Z. Cui
    • 2
  •  Z. Huang
    • 3
  •  M. Li
    • 2
  •  M. Ishii
    • 1
  •  Y. Igarashi
    • 1
  1. 1.Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
  2. 2.Department of Agronomy, China Agricultural University, Yuanmingyuan West Road, Haidian District, Beijing 100094, P.R. China
  3. 3.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China

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