Applied Microbiology and Biotechnology

, Volume 59, Issue 2–3, pp 289–296 | Cite as

Effect of glucose on glycerol bioconversion by Lactobacillus reuteri

  •  Q. Lüthi-Peng
  •  F. Dileme
  •  Z. Puhan
Original Paper

Abstract.

The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS (modified MRS – omitting glucose, acetate and Tween 80) and distilled water (H2O). In MRS, addition of glucose enhanced glycerol metabolism in resting cells of L. reuteri, consequently increasing the accumulation of 3-HPA by regulating the NAD/NADH ratio. Enhanced glycerol metabolism correlated positively with the concentration of glucose. NADH produced during glucose metabolism was preferentially reoxidized to NAD by the reduction of 3-HPA to 1,3-propanediol; an adequate supply of glycerol therefore outweighed the repression of glucose on the accumulation of 3-HPA. At a molar ratio of glucose to glycerol no greater than 0.33, accumulation of 3-HPA was favored. In non-growing medium (H2O), addition of glucose seemed to be counter-productive with respect to 3-HPA accumulation. Lactate had a positive impact on glycerol metabolism, presumably by altering the redox flux, resulting in enhanced 3-HPA accumulation in both MRS and H2O systems.

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  Q. Lüthi-Peng
    • 1
  •  F. Dileme
    • 1
  •  Z. Puhan
    • 1
  1. 1.Laboratory of Dairy Science, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology Zürich, 8092 Zürich, Switzerland

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