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Applied Microbiology and Biotechnology

, Volume 59, Issue 2–3, pp 289–296 | Cite as

Effect of glucose on glycerol bioconversion by Lactobacillus reuteri

  •  Q. Lüthi-Peng
  •  F. Dileme
  •  Z. Puhan
Original Paper

Abstract.

The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS (modified MRS – omitting glucose, acetate and Tween 80) and distilled water (H2O). In MRS, addition of glucose enhanced glycerol metabolism in resting cells of L. reuteri, consequently increasing the accumulation of 3-HPA by regulating the NAD/NADH ratio. Enhanced glycerol metabolism correlated positively with the concentration of glucose. NADH produced during glucose metabolism was preferentially reoxidized to NAD by the reduction of 3-HPA to 1,3-propanediol; an adequate supply of glycerol therefore outweighed the repression of glucose on the accumulation of 3-HPA. At a molar ratio of glucose to glycerol no greater than 0.33, accumulation of 3-HPA was favored. In non-growing medium (H2O), addition of glucose seemed to be counter-productive with respect to 3-HPA accumulation. Lactate had a positive impact on glycerol metabolism, presumably by altering the redox flux, resulting in enhanced 3-HPA accumulation in both MRS and H2O systems.

Keywords

Glucose Acetate Glycerol Lactate NADH 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  Q. Lüthi-Peng
    • 1
  •  F. Dileme
    • 1
  •  Z. Puhan
    • 1
  1. 1.Laboratory of Dairy Science, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology Zürich, 8092 Zürich, Switzerland

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