A continuous lactic acid production system using an immobilized packed bed of Lactobacillus helveticus
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A 5 l packed bed bioreactor was used to study the effect of initial lactose concentration and hydraulic retention time (HRT) on cell growth, lactose utilization and lactic acid production. Up to 95% of the initial lactose concentration was utilized at longer HRTs (30–36 h). The study showed that lactic acid production increased with increases in HRT (12–36 h) and initial lactose concentrations. The highest lactic acid production rate (3.90 g l–1 h–1) was obtained with an initial lactose concentration of 100 g/l and an HRT of 18 h, whereas the lowest lactic acid production rate (1.35 g l–1 h–1) was obtained with an initial lactose concentration of 50 g/l and an HRT of 36 h. This suggested that optimal lactic acid production can be achieved at an HRT of 18 h and initial lactose concentration of 100 g/l.
KeywordsLactic Acid Lactose Lactobacillus Hydraulic Retention Time Lactic Acid Production
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