Applied Microbiology and Biotechnology

, Volume 58, Issue 2, pp 164–169 | Cite as

Polygalacturonase production by Aspergillus awamori on wheat in solid-state fermentation

  •  A. Blandino
  •  T. Iqbalsyah
  •  S. Pandiella
  •  D. Cantero
  •  C. Webb
Original Paper

Abstract.

The production of exo-polygalacturonase (exo-PG) and endo-PG by Aspergillus awamori grown on wheat in solid-state fermentation was studied. Endo- and exo-PG activities were detected after 24 h of inoculation. Glucose released from starch hydrolysis acted as a catabolite repressor for the exo-PG enzyme. In contrast, endo-PG production was not affected by glucose repression. When milled grains were used, the particle-size distribution and the chemical composition of the medium influenced the rate of micro-organism growth and therefore the trend followed by endo- and exo-PG production. However, these two parameters did not affect the maximum production of exo-PG and endo-PG. For one of the milled samples, three different moisture contents were used (50, 55, 60%). Moisture contents of 60% provide a higher yield of pectinases by A. awamori.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  A. Blandino
    • 2
  •  T. Iqbalsyah
    • 3
  •  S. Pandiella
    • 1
  •  D. Cantero
    • 2
  •  C. Webb
    • 1
  1. 1.Satake Centre for Grain Process Engineering, Department of Chemical Engineering, UMIST, P.O. Box 88, Manchester M60 1QD, UK
  2. 2.Department of Chemical Engineering, Faculty of Sciences, University of Cádiz, Polígono Río San Pedro, Puerto Real, 11510 Cadiz, Spain
  3. 3.Faculty of Mathematics and Science, Syiah Kuala University, Kampus Unsyiah, Darussalam – Banda Aceh 23111, Indonesia

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