Applied Microbiology and Biotechnology

, Volume 58, Issue 1, pp 63–66

Production of teicoplanin by valine analogue-resistant mutant strains of Actinoplanes teichomyceticus

  •  Z. Jin
  •  M. Wang
  •  P. Cen
Original Paper

Abstract.

Teicoplanin is a glycopeptide antibiotic produced by Actinoplanes teichomyceticus. A strain improvement to increase the productivity of the major component, teicoplanin A2-2, was carried out. As the fatty moiety of teicoplanin A2-2 is derived from L-valine, L-valine analogue (valine hydroxamate)-resistant mutants were derived. One of the mutants, 98-1-227, overproduced valine and produced a higher titer of total teicoplanin with higher A2-2 content. In a pilot fermentor (7 m3), the total productivity of teicoplanin was 1,800 units/ml and the A2-2 content was 58%.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Z. Jin
    • 1
  •  M. Wang
    • 2
  •  P. Cen
    • 1
  1. 1.Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P. R. China
  2. 2.Sichuan Industrial Institute of Antibiotics, Chengdu 610051, P. R. China

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