Immunogenetics

, Volume 49, Issue 11–12, pp 949–956 | Cite as

Genetic mapping of the embryonal carcinoma transplantation resistance locus Gt(B6) to mouse Chromosome 8

  • Alexander J. Muller
  • Katherine B. Heiden
  • Angelika K. Teresky
  • A. J. Levine
  • K. B Heiden
ORIGINAL PAPER

Abstract

 Cytotoxic T lymphocytes play a predominant role in allograft rejection. They mediate this process through recognition of foreign major histocompatibility complex (MHC) class I surface molecules encoded at the H2 locus. Embryonal carcinoma cells, the undifferentiated, neoplastic derivatives of primordial germ cells, typically lack detectable MHC class I gene expression. Despite this, embryonal carcinoma cells are subject to allograft rejection in several different mouse strains. In many instances, the H2 locus appears to be genetically linked to resistance. However, rejection of allografts of the F9 embryonal carcinoma cell line, a nullipotent cell line derived from the 129 mouse strain, does not appear to be H2-linked. Resistance to F9 tumor formation in the C57BL/6 mouse strain has been attributed to a single, unidentified locus termed Gt(B6). To genetically map the Gt(B6) locus, a total of 463 (C57BL/6×129)F2 mice were challenged with F9 cells, and 78 tumor-resistant mice were identified. Markers encompassing two candidate regions, the H2 locus on Chromosome (Chr) 17 and a second candidate locus on Chr 2, showed no indication of linkage to the resistance phenotype. Instead, results of a genome wide scan implicated mouse Chr 8, and evidence is presented demonstrating that the Gt(B6) locus maps to a region of less than 10 cM on the medial portion of Chr 8.

Key words Transplantation Histocompatibility Embryonal carcinoma Teratocarcinoma Mouse Chromosome 8 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Alexander J. Muller
    • 1
  • Katherine B. Heiden
  • Angelika K. Teresky
    • 1
  • A. J. Levine
    • 3
  • K. B Heiden
    • 2
  1. 1.Department of Molecular Biology, Lewis Thomas Laboratory, Princeton, University, Princeton, NJ 08544, USAUS
  2. 2.College of Physicians and Surgeons, Columbia University, 2960 Broadway, New York, NY 10027, USAUS
  3. 3.Office of the President, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA e-mail: alevine@rockvax.rockefeller.edu, Tel.: +1-212-3278134, Fax: +1-212-3278505US

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