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Immunogenetics

, Volume 49, Issue 5, pp 384–394 | Cite as

Gene organization of the quail major histocompatibility complex (MhcCoja) class I gene region

  • Takashi Shiina
  • Chiori Shimizu
  • Akira Oka
  • Yoshika Teraoka
  • Tadashi Imanishi
  • Takashi Gojobori
  • K. Hanzawa
  • Seiki Watanabe
  • H. Inoko
ORIGINAL PAPER

Abstract

 Class I genomic clones of the quail (Coturnix japonica) major histocompatibility complex (MhcCoja) were isolated and characterized. Two clusters spanning the 90.8 kilobase (kb) and 78.2 kb class I gene regions were defined by overlapping cosmid clones and found to contain at least twelve class I loci. However, unlike in the chicken Mhc, no evidence for the existence of any Coja class II gene was obtained in these two clusters. Based on comparative analysis of the genomic sequences with those of the cDNA clones, Coja-A, Coja-B, Coja-C, and Coja-D (Shiina et al. 1999), these twelve loci were assigned to represent one Coja-A gene, two Coja-B genes (Coja-B1 and -B2), four Coja-C genes (Coja-C1-C4), four Coja-D genes (Coja-D1-D4), and one new Coja-E gene. A class I gene-rich segment of 24.6 kb in which five of these genes (Coja-B1, -B2, -D1, -D2 and -E) are densely packed were sequenced by the shotgun strategy. All of these five class I genes are very compact in size [2089 base pairs (bp)–2732 bp] and contain no apparent genetic defect for functional expression. A transporter associated with the antigen processing (TAP) gene was identified in this class I gene-rich segment. These results suggest that the quail class I region is physically separated from the class II region and characterized by a large number of the expressible class I loci (at least seven) in contrast to the chicken Mhc, where the class I and class II regions are not clearly differentiated and only at most three expressed class I loci so far have been recognized.

Key words Antigen processing Evolution Cell surface molecules Mhc Transporters 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Takashi Shiina
    • 1
  • Chiori Shimizu
    • 3
  • Akira Oka
    • 1
  • Yoshika Teraoka
    • 1
  • Tadashi Imanishi
    • 4
  • Takashi Gojobori
    • 4
  • K. Hanzawa
    • 2
  • Seiki Watanabe
    • 2
  • H. Inoko
    • 1
  1. 1.Department of Genetic Information, Division of Molecular Life Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan, E-mail: hiniko@ig.icc.u-tokai.ac.jp, Tel.: +81-463-931121; Fax: +81-463-948884JP
  2. 2.Laboratory of Animal Physiology, Department of Zootechnical Science, Tokyo University of Agriculture, Sakuragaoka, Setagayaku, Tokyo 156-0054, JapanJP
  3. 3.Division of Molecular Immunology, Center for Biomedical Sciences, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-0856, JapanJP
  4. 4.DNA research center, National Institute of Genetics, Mishima, Shizuoka 411-0801, JapanJP

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