, Volume 49, Issue 2, pp 106–114 | Cite as

Evolution of the arthropod prophenoloxidase/hexamerin protein family

  • Austin L. Hughes


 Phylogenetic analysis of the prophenoloxidase/hexamerin family of arthropods revealed four well supported subfamilies: (1) the arylphorin subfamily, including arylphorins, storage proteins, and other proteins of uncertain function from insects; (2) the hemocyanins of branchiopod crustaceans, which are copper-binding proteins involved in oxygen transport; (3) the hemocyanins of chelicerates; and (4) the prophenoloxidases (proPO) of both insects and branchiopods, which are copper-binding molecules that play a role in sclerotization of cuticle and encapsulation of foreign particles. The phylogeny indicated that insect and branchiopod proPO constitute a monophyletic group but that branchiopod and chelicerate hemocyanins do not constitute a monophyletic group. Branchiopod hemocyanin and proPO diverged from each other prior to the divergence of insects from branchiopods and probably prior to the divergence of chelicerates from the insect-branchiopod lineage. Likewise, the insect arylphorin subfamily diverged from proPO prior to the divergence of insects from branchiopods and probably prior to the divergence of chelicerates; thus, the results did not support the hypothesis that insect arylphorins represent hemocyanins freed to assume a new function because the insect tracheal respiratory system removes the need for an oxygen-transport molecule. Nonetheless, reconstruction of ancestral sequences by the maximum parsimony method suggested that the ancestors of the arylphorin family were copper-binding. Regions corresponding to the copper-binding domains were found to have a faster rate of nonsynonymous evolution in arylphorin subfamily genes than in other hexamerin family genes; this presumably reflects a relaxation of purifying selection after the loss of copper-binding function.

Key words Arylphorin Hexamerin Invertebrate immunity Propheroloxidase 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Austin L. Hughes
    • 1
  1. 1.Department of Biology and Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16803, USA, E-mail:, Tel.: +1-814-865-5013, Fax: +1-814-865-9131US

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