Immunogenetics

, Volume 47, Issue 5, pp 398–403

Tyrosinase epitope recognized by an HLA-DR-restricted T-cell line from a Vogt-Koyanagi-Harada disease patient

  • H. Kobayashi
  • T. Kokubo
  • Michinari Takahashi
  • Keisuke Sato
  • Naoyuki Miyokawa
  • Shoji Kimura
  • Reiko Kinouchi
  • Makoto Katagiri
ORIGINAL PAPER

DOI: 10.1007/s002510050375

Cite this article as:
Kobayashi, H., Kokubo, T., Takahashi, M. et al. Immunogenetics (1998) 47: 398. doi:10.1007/s002510050375

Abstract

 Human T-cell-mediated autoimmune diseases are often genetically linked to particular alleles of HLA class II genes. Vogt-Koyanagi-Harada’s (VKH) disease, which is regarded as an autoimmune disorder in multiple organs containing melanocytes, has been found to be associated with HLA-DR4 (DRB1*0405) and HLA-DR53 (DRB4*0101). Tyrosinase is a melanoma antigen (Ag) expressed by normal melanocytes as well as melanoma cells against which responses by autologous T cells have been detected. We established a T-cell line from the peripheral blood of a patient with VKH disease which responded to synthetic peptides corresponding to tyrosinase. The T-cell line was generated which recognized the tyrosinase p188 – 208 peptide when presented by the HLA-DR4 (DRB1*0405) molecule on the surface of HLA class II-expressing L-cell transfectants. The minimal antigenic peptide which induced T-cell responses was an 11-amino-acid sequence and located at tyrosinase p193 – 203 (E-I-W-R-D-I-D-F-A-H-E). This peptide contained the DRB1*0405-binding peptide motif (hydrophobic residues (Y, F, W) at position 1 as an anchor residue, and negatively charged residues (D, E) at position 9), which corresponded to the W at p195 and the D at p203. These observations demonstrate that tyrosinase peptides are immunogenic, and may be a candidate for an autoantigen in VKH disease, suggesting that probing the T-cell responses against synthetic peptides is a productive approach for identifying the autoantigenic peptides associated with autoimmune diseases including VKH disease.

Key words HLA Peptide VKH disease Tyrosinase T-cell response 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. Kobayashi
    • 1
  • T. Kokubo
    • 1
  • Michinari Takahashi
    • 1
  • Keisuke Sato
    • 1
  • Naoyuki Miyokawa
    • 1
  • Shoji Kimura
    • 1
  • Reiko Kinouchi
    • 1
  • Makoto Katagiri
    • 1
  1. 1.Department of Pathology, Asahikawa Medical College, 4-5-3-11, Nishikagura, Asahikawa, 078, JapanJP

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