Immunogenetics

, Volume 45, Issue 5, pp 285–294

Isolation of Mhc class I cDNAs from the axolotl Ambystoma mexicanum

  • Bénédicte Sammut
  • Véronique Laurens
  • A. Tournefier
ORIGINAL PAPER

DOI: 10.1007/s002510050207

Cite this article as:
Sammut, B., Laurens, V. & Tournefier, A. Immunogenetics (1997) 45: 285. doi:10.1007/s002510050207

Abstract

 Class I major histocompatibility complex (Mhc) cDNA clones were isolated from axolotl mRNA by polymerase chain reaction (PCR) and by screening a cDNA phage library. The nucleotide and predicted amino acid sequences show definite similarities to the Mhc class Iα molecules of higher vertebrates. Most of the amino acids in the peptide binding region that dock peptides at their N and C termini in mammals are conserved. Several amino acids considered to be important for the interaction of β2-microglobulin with the Mhc α chain are also conserved in the axolotl sequence. The fact that axolotl class I A cDNAs are ubiquitously expressed and highly polymorphic in the α1 and α2 domains suggests the classical nature of axolotl class I A genes.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Bénédicte Sammut
    • 1
  • Véronique Laurens
    • 1
  • A. Tournefier
    • 1
  1. 1.Laboratoire d’Immunologie Comparée, CNRS-UMR 5548, Université de Bourgogne, 6 Boulevard Gabriel, 21000 Dijon, FranceFR

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