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Immunogenetics

, Volume 45, Issue 4, pp 266–273 | Cite as

Isolation of a B-cell-specific promoter for the human class II transactivator

  • Ana-Maria Lennon
  • Catherine Ottone
  • Gildas Rigaud
  • Larry L. Deaven
  • Jon Longmire
  • Marc Fellous
  • Rosa Bono
  • C. Alcaïde-Loridan
ORIGINAL PAPER

Abstract

 The class II transactivator (CIITA) is essential for the expression of major histocompatibility complex (MHC) class II antigens. The tissular patterns of CIITA and MHC class II gene expression are tightly correlated: CIITA mRNA is highly expressed in B cells, and is induced by interferon gamma (IFN-γ) in macrophage and epithelial cell lines. We first isolated two overlapping cosmids encoding human CIITA which, when co-transfected, are able to restore MHC class II expression in a B-lymphoblastoid cell line (B-LCL) defective for CIITA. Subsequently, a 1.8 kilobase (kb) fragment of the CIITA promoter was isolated and sequenced. A motif presenting a strong similarity to an initiator was detected, as well as putative binding sites for Sp1, GATA-2, LyF-1, ets-1, AP1, and MZF1 transcription factors, and two GAS motifs. When introduced in front of a luciferase reporter gene, this promoter is able to direct a high luciferase activity in a human B-LCL. In contrast, luciferase expression was not stimulated after IFN-γ treatment when the construct was transfected in macrophage or in epithelial cell lines. However, an induction of the human CIITA gene was observed in mouse macrophage and fibrosarcoma cell lines, when the cells were transfected with a cosmid containing the human CIITA gene, but lacking the 1.8 kb promoter described above. Taken together, these data suggest the existence of an intragenic promoter driving an IFN-γ-inducible expression of CIITA.

Keywords

Major Histocompatibility Complex Major Histocompatibility Complex Class Epithelial Cell Line Interferon Gamma Fibrosarcoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Ana-Maria Lennon
    • 1
  • Catherine Ottone
    • 1
  • Gildas Rigaud
    • 2
  • Larry L. Deaven
    • 3
  • Jon Longmire
    • 3
  • Marc Fellous
    • 1
  • Rosa Bono
    • 4
  • C. Alcaïde-Loridan
    • 1
  1. 1.Unité d’Immunogénétique Humaine, INSERM U276, Institut Pasteur. 25, rue du Dr. Roux, 75724 Paris cedex 15, FranceFR
  2. 2.Unité d’Immunogénétique, INSERM U276, Institut Pasteur, Paris, FranceFR
  3. 3.Center for human Genome Studies, Los Alamos National Laboratory, New Mexico, USAMX
  4. 4.Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, ChileCL

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