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Immunogenetics

, Volume 70, Issue 1, pp 37–51 | Cite as

Variants in the non-coding region of the TLR2 gene associated with infectious subphenotypes in pediatric sickle cell anemia

  • Susana David
  • Pedro Aguiar
  • Liliana Antunes
  • Alexandra Dias
  • Anabela Morais
  • Anavaj Sakuntabhai
  • João Lavinha
Original Article
  • 249 Downloads

Abstract

Sickle cell anemia (SCA) is characterized by chronic hemolysis, severe vasoocclusive crises (VOCs), and recurrent often severe infections. A cohort of 95 SCA pediatric patients was the background for genotype-to-phenotype association of the patient’s infectious disease phenotype and three non-coding polymorphic regions of the TLR2 gene, the −196 to −174 indel, SNP rs4696480, and a (GT)n short tandem repeat. The infectious subphenotypes included (A) recurrent respiratory infections and (B) severe bacterial infection at least once during the patient’s follow-up. The absence of the haplotype [Del]-T-[n ≥ 17] (Hap7) in homozygocity protected against subphenotype (B), in a statistically significant association, resisting correction for multiple testing. For the individual loci, the same association tendencies were observed as in the haplotype, including a deleterious association between the SNP rs4696480 T allele and subphenotype (A), whereas the A/A genotype was protective, and a deleterious effect of the A/T genotype with subphenotype (B), as well as including the protective effect of −196 to −174 insert (Ins) and deleterious effect of the deletion (Del) in homozygocity, against subphenotype (B). Moreover, a reduction in the incidence rate of severe bacterial infection was associated to a rise in the hemolytic score, fetal hemoglobin levels (prior to hydroxyurea treatment), and 3.7-kb alpha-thalassemia. Interestingly, differences between the effects of the two latter covariables favoring a reduction in the incidence rate of subphenotype (B) contrast with a resulting increase in relation to subphenotype (A). These results could have practical implications in health care strategies to lower the morbidity and mortality of SCA patients.

Keywords

Sickle cell anemia TLR2 Genetic variants Viral and bacterial infection Hemolytic component Genotype-to-phenotype association 

Notes

Acknowledgements

We are grateful to the patients and their families. We also thank Dominique Labie for having suggested this topic of research. We thank INSA’s “Unidade de Tecnologia e Inovacão” as well as Andreia Coelho and Emanuel Ferreira for their technical support. We thank Eric David Bosne for the insight in PCA. We also thank Audrey V. Grant, Baltazar Nunes, Paula Faustino, and Vera C.M. David for their helpful suggestions in the elaboration of the study. This study was carried out with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020, in the project with reference UIDMULTI/00211/2013, and was partially funded by FCT grants PIC/IC/83084/2007 and the Centro de Investigação em Genética Molecular Humana (CIGMH).

Author contribution

Conceived and designed the experiments: S David, A Dias, A Morais, J Lavinha. Suggested methodologies: S David, A Dias, A Morais, A Sakuntabhai, J Lavinha. Genotyped: S David. Reorganized the database: S David. Analyzed the data: S David, P Aguiar. Carried out the investigation: S David. Provided the resources: A Dias, A Morais, J Lavinha. Wrote the paper: S David. Reviewed the paper: P Aguiar, J Lavinha, A Sakuntabhai. All authors approved the final version of the paper.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Supplementary material

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Fig. S1 (PPTX 65 kb)
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Fig. S2 (PPTX 77 kb)
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Table S1 (DOCX 25 kb)
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Table S2 (DOCX 35 kb)
251_2017_1013_MOESM5_ESM.docx (41 kb)
Table S3 (DOCX 40 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Susana David
    • 1
    • 2
  • Pedro Aguiar
    • 3
  • Liliana Antunes
    • 4
  • Alexandra Dias
    • 5
  • Anabela Morais
    • 6
  • Anavaj Sakuntabhai
    • 7
    • 8
  • João Lavinha
    • 1
    • 9
  1. 1.Departamento de Genética HumanaInstituto Nacional de Saúde Doutor Ricardo JorgeLisbonPortugal
  2. 2.Centro de Estudos de Ciência Animal, Instituto de Ciências e Tecnologias Agrárias e Agro-AlimentaresUniversidade do PortoPortoPortugal
  3. 3.Centro de Investigação em Saúde Pública, Escola Nacional de Saúde PublicaUniversidade Nova de LisboaLisbonPortugal
  4. 4.Departamento de EpidemiologiaInstituto Nacional de Saúde Doutor Ricardo JorgeLisbonPortugal
  5. 5.Departamento de PediatriaHospital Prof Doutor Fernando FonsecaAmadoraPortugal
  6. 6.Departamento de PediatriaHospital de Santa MariaLisbonPortugal
  7. 7.Unité de la Génétique Fonctionnelle des Maladies InfectieusesInstitut, PasteurParisFrance
  8. 8.Centre National de la Recherche ScientifiqueParisFrance
  9. 9.BioISI, Faculdade de CiênciasUniversidade de LisboaLisbonPortugal

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