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Immunogenetics

, Volume 68, Issue 5, pp 339–352 | Cite as

Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters

  • Isaiah ObaraEmail author
  • Morten Nielsen
  • Marie Jeschek
  • Ard Nijhof
  • Camila J. Mazzoni
  • Nicholas Svitek
  • Lucilla Steinaa
  • Elias Awino
  • Cassandra Olds
  • Ahmed Jabbar
  • Peter-Henning Clausen
  • Richard P. Bishop
Original Article

Abstract

There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.

Keywords

Theileria parva CD8+ T cell epitopes Class I MHC Ankole cattle Pyrosequencing 

Notes

Acknowledgments

We are grateful to Dr. Joerg Jores of ILRI for reviewing the manuscript. We also wish to thank Giles Prettejohn of the Ol Pejeta Conservancy in Kenya whose efforts were instrumental to the completion of this study. The study was funded by the DFG German-African Cooperation Projects in Infectiology: ‘Molecular epidemiology network for promotion and support of delivery of life vaccines against Theileria parva and Theileria annulata infection in Eastern and Northern Africa (SE 862/2-1)’. Some of the work described in this paper was also supported by the CGIAR Consortium research project CRP3.7.

Supplementary material

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Supplementary Fig. 1 (DOCX 29 kb)
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Supplementary Fig. 2 (DOCX 31 kb)
251_2016_902_MOESM3_ESM.docx (82 kb)
Supplementary Table 1 (DOCX 82 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Isaiah Obara
    • 1
    Email author
  • Morten Nielsen
    • 2
    • 3
  • Marie Jeschek
    • 4
  • Ard Nijhof
    • 1
  • Camila J. Mazzoni
    • 4
    • 5
  • Nicholas Svitek
    • 6
  • Lucilla Steinaa
    • 6
  • Elias Awino
    • 6
  • Cassandra Olds
    • 6
  • Ahmed Jabbar
    • 1
  • Peter-Henning Clausen
    • 1
  • Richard P. Bishop
    • 6
  1. 1.Institute for Parasitology and Tropical Veterinary MedicineFreie Universität BerlinBerlinGermany
  2. 2.Center for Biological Sequence Analysis (CBS), Department of Systems BiologyThe Technical University of DenmarkLyngbyDenmark
  3. 3.Instituto de Investigaciones BiotecnológicasUniversidad Nacional de San MartínSan MartínArgentina
  4. 4.Berlin Center for Genomics in Biodiversity Research (BeGenDiv)BerlinGermany
  5. 5.Leibniz-Institute for Zoo and Wildlife Research (IZW)Evolutionary GeneticsBerlinGermany
  6. 6.International Livestock Research Institute (ILRI)NairobiKenya

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