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Immunogenetics

, Volume 68, Issue 1, pp 43–54 | Cite as

miR-27a and miR-214 exert opposite regulatory roles in Th17 differentiation via mediating different signaling pathways in peripheral blood CD4+ T lymphocytes of patients with relapsing–remitting multiple sclerosis

  • Maryam Ahmadian-Elmi
  • Ali Bidmeshki Pour
  • Reza Naghavian
  • Kamran Ghaedi
  • Somayeh Tanhaei
  • Tayebeh Izadi
  • Mohammad Hossein Nasr-Esfahani
Original Paper

Abstract

Multiple sclerosis (MS) is one of the most prevalent autoimmune diseases, which involves the central nervous system. In this illness, Treg/Th17 cell imbalance causes the defect. Several studies revealed that T helper 17 (Th17) cells play a crucial role in pathogenesis, inflammation, and autoimmunity of several autoimmune diseases such as MS. In the present study, we assessed transcript levels of miR-27a and miR-214, in purified CD4+ T cells of MS patients, during relapsing and remitting phases in inducing differentiation of T naïve cells to Th17 cells. Forty RR-MS patient samples including those in relapsing (n = 20) and remitting (n = 20) phases were participated in this study. In addition, transcript levels of IL-17A, RORγt, IL-23R, Foxp3, and TGF-β in purified CD4+ T cells of patients in relapsing and remitting phases of RRMS patients were compared to healthy controls. Expression levels of miR-27a and miR-214 were measured by RT-qPCR and compared to healthy control group (n = 10). Data indicated upregulation of miR27a in relapsing phase of multiple sclerosis compared to remitting phase and healthy volunteers while miR-214 downregulated in relapsing phase of MS compared to remitting phase and healthy volunteers. In silico studies demonstrated pathways which miR-27a and miR-214 could effect on CD4+ T cell lineage fate including TGF-β and mTOR signaling, respectively. Our data suggest that miR-27a may probably inhibit negative regulators of Th17 cell differentiation, thus promoting its differentiation while miR-214 has an adverse effect.

Keywords

miR-27a miR-214 Th17 cells miRNA Differentiation Relapsing–remitting multiple sclerosis 

Notes

Acknowledgments

We thank the blood donors, Al-Zahra Hospital staffs, and our colleagues especially the MS research team in Royan Institute for their helpful participation and comments. All authors support submission to this journal.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

251_2015_881_Fig8_ESM.gif (60 kb)
Fig. S1

Real-time PCR profiles and gel electrophoresis patterns. a. b. c Real-time PCR profiles for miR-27a, miR-214, and RNU48. d Real-time PCR products for miR-27a, miR-214, and RNU48 were run on 12 % polyacrylamide gel to evaluate the specificity of primers used (M 50 bp) (GIF 60 kb)

251_2015_881_MOESM1_ESM.tif (3 mb)
High Resolution Image (TIF 3102 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Biology, Faculty of ScienceRazi UniversityKermanshahIran
  2. 2.Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for BiotechnologyACECRIsfahanIran
  3. 3.Division of Cellular and Molecular Biology, Department of Biology, Faculty of SciencesUniversity of IsfahanIsfahanIran

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