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Immunogenetics

, Volume 65, Issue 5, pp 345–355 | Cite as

Allelic variants in TLR10 gene may influence bilateral affectation and clinical course of Meniere’s disease

  • Teresa Requena
  • Irene Gazquez
  • Antonia Moreno
  • Angel Batuecas
  • Ismael Aran
  • Andres Soto-Varela
  • Sofia Santos-Perez
  • Nicolas Perez
  • Herminio Perez-Garrigues
  • Alicia Lopez-Nevot
  • Eduardo Martin
  • Ricardo Sanz
  • Paz Perez
  • Gabriel Trinidad
  • Marta E. Alarcon-Riquelme
  • Roberto Teggi
  • Laura Zagato
  • Miguel A. Lopez-Nevot
  • Jose A. Lopez-EscamezEmail author
Original Paper

Abstract

Toll-like receptors trigger the innate immune response by activating various cell types such us macrophages and lymphocytes. We genotyped SNV of TLR3, TRL7, TLR8 and TLR10 in 863 Spanish and 150 Italian patients with Meniere’s disease (MD) and 1,013 controls by using Taqman assays. Real-Time qPCR was used to measure the expression level of TLR10 in peripheral blood leukocytes. The overall dataset showed that the C allele and the CC genotype of rs11096955 in TLR10 gene were more commonly observed in controls than patients (corrected p = 1 × 10−3, OR = 0.68 [95 % confidence interval, 0.54–0.84] for CC genotype; corrected p = 1.5 × 10−5, OR = 0.75 [0.66–0.85] for allele C). Moreover, the CC genotype was more frequent in patients with uni- (19 %) than bilateral sensorineural hearing loss (SNHL) (13 %). Logistic regression demonstrated that the time since the onset of MD, Tumarkin crises, hearing stage and rs11096955 were independent factors influencing the risk of bilateral SNHL. In addition, rs11096955 influenced hearing loss progression in patients with bilateral MD. No change in expression of TLR10 was observed according to CC, CA or AA genotypes. Our data suggest that allelic variants of TLR10 gene may influence the susceptibility and time-course of hearing loss of MD in the European population.

Keywords

Mènière’s disease Toll-like receptor Single-nucleotide polymorphisms Sensorineural hearing loss 

Notes

Acknowledgments

We would like to thank all MD patients and control subjects for participating in this study. We thank Ana Anton Conejero, Maria Antonia Gallego and Cristobal Zapata for the help in the sample recruitment and Casimiro Castillejo-Lopez and Alejandro Diaz Barreiro for their assistance with the RNA isolation. This work was funded by Research Grants FIS PI09/00920 from Instituto de Salud Carlos III and BIO103SA/51/11 from Health Department of the Castilla y Leon Regional Government by FEDER Funds from the EU. JALE was partially supported by DP-0041-2011 Grant from Health Department of Andalusian Regional Government.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Teresa Requena
    • 1
  • Irene Gazquez
    • 1
    • 2
  • Antonia Moreno
    • 2
  • Angel Batuecas
    • 3
  • Ismael Aran
    • 4
  • Andres Soto-Varela
    • 5
  • Sofia Santos-Perez
    • 5
  • Nicolas Perez
    • 6
  • Herminio Perez-Garrigues
    • 7
  • Alicia Lopez-Nevot
    • 8
  • Eduardo Martin
    • 9
  • Ricardo Sanz
    • 9
  • Paz Perez
    • 10
  • Gabriel Trinidad
    • 11
  • Marta E. Alarcon-Riquelme
    • 12
  • Roberto Teggi
    • 13
  • Laura Zagato
    • 14
  • Miguel A. Lopez-Nevot
    • 2
  • Jose A. Lopez-Escamez
    • 1
    • 15
    Email author
  1. 1.Otology and Neurotology Group CTS495, Human DNA Variability Department, Centro de Genómica e Investigación OncológicaPfizer/Universidad de Granada/Junta de Andalucía (GENYO)GranadaSpain
  2. 2.Department of ImmunologyHospital Virgen de las NievesGranadaSpain
  3. 3.Department of OtolaryngologyHospital Universitario de SalamancaSalamancaSpain
  4. 4.Department of OtolaryngologyComplexo Hospitalario de PontevedraPontevedraSpain
  5. 5.Division of Otoneurology, Department of OtorhinolaryngologyComplexo Hospitalario UniversitarioSantiago de CompostelaSpain
  6. 6.Department of OtolaryngologyClinica Universidad de NavarraPamplonaSpain
  7. 7.Division Otoneurology, Department of OtorhinolaryngologyHospital La FeValenciaSpain
  8. 8.Department of OtolaryngologyHospital Virgen de las NievesGranadaSpain
  9. 9.Department OtolaryngologyHospital Universitario de GetafeMadridSpain
  10. 10.Department of OtolaryngologyHospital de CabueñesGijónSpain
  11. 11.Department of OtorhinolaryngologyComplejo Hospitalario BadajozBadajozSpain
  12. 12.Group of Genetics of Complex Diseases, Human DNA Variability Department, Centro de Genómica e Investigación OncológicaPfizer/Universidad de Granada/Junta de Andalucía (GENYO)GranadaSpain
  13. 13.Department of OtolaryngologySan Raffaelle Scientific InstituteMilanItaly
  14. 14.Division of Dialysis and NephrologyHospital San Raffaelle Scientic InstituteMilanItaly
  15. 15.Department of OtolaryngologyHospital de PonienteAlmeriaSpain

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