Study of cynomolgus monkey (Macaca fascicularis) Mhc DRB gene polymorphism in four populations
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The cynomolgus macaque (Macaca fascicularis) is currently used as an animal model in various fields of immunology especially in the development of innovative vaccines for the prevention and treatment of infectious diseases. The polymorphism of the major histocompatibility complex (MHC) influences the development of adaptive immune responses, and it is crucial to characterize the polymorphism of cynomolgus MHC genes. Among all macaque species, the cynomolgus macaque has the most diversified geographical area encompassing continental and insular populations. By the study of a large sample of animals from the Philippines (N = 359), we have characterized 20 DRB haplotypes. The DRB genotyping was performed by denaturing gradient gel electrophoresis (DGGE) sequencing of exon 2 and was confirmed by polymerase chain reaction–sequence-specific oligonucleotide. The DRB and DRA cDNA of 126 animals were characterized by cloning and sequencing. By means of DGGE sequencing, we characterized the polymorphism of genomic DRB exon 2 in three other cynomolgus macaque population samples (Java, Vietnam, and Mauritius), and we discuss about the origin of the founders of the Mauritian and the Filipino cynomolgus macaque populations.
KeywordsMHC DRB Cynomolgus macaque Polymorphism
We are pleased to thank all the technicians of the Toulouse Laboratory of Immunogenetics for their excellent technical assistance: Béatrice Atlan, Audrey Dauba, Stéphanie Despiau-Schiavinato, and Sylvie Hébrard. We thank Kouji Otabe, Kengo Sakamoto, Masaaki Kurata, and Mamoru Nomura for helping with the cynomolgus macaque blood collection. This work was supported by Grant-in-Aid for Scientific Research (B) (21300155) from Japan Society for the Promotion of Science (JSPS) and by a grant from the French Ministry of Research (contract EA3034) and a grant from University Paul Sabatier Toulouse 3.
Conflict of interest
The authors declare that they have no conflict of interest.
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