Diversity of MHC class I haplotypes in cynomolgus macaques
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Cynomolgus macaques are widely used as a primate model for human diseases associated with an immunological process. Because there are individual differences in immune responsiveness, which are controlled by the polymorphic nature of the major histocompatibility (MHC) locus, it is important to reveal the diversity of MHC in the model animal. In this study, we analyzed 26 cynomolgus macaques from five families for MHC class I genes. We identified 32 Mafa-A, 46 Mafa-B, 6 Mafa-I, and 3 Mafa-AG alleles in which 14, 20, 3, and 3 alleles were novel. There were 23 MHC class I haplotypes and each haplotype was composed of one to three Mafa-A alleles and one to five Mafa-B alleles. Family studies revealed that there were two haplotypes which contained two Mafa-A1 alleles. These observations demonstrated further the complexity of MHC class I locus in the Old World monkey.
KeywordsCynomolgus macaque MHC Mafa class I gene Haplotype Polymorphism
We thank Ms. Yukiko Ueda for her technical assistance. We acknowledge Dr. Natasja de Groot and Dr. Nel Otting for assigning nomenclature of Mafa class I alleles. This work was supported in part by research grants from the Ministry of Health, Labor and Welfare, Japan; the Japan Health Science Foundation; the program of Founding Research Centers for Emerging and Reemerging Infection Disease; the program of Research on Publicly Essential Drugs and Medical Devices; and Grant-in-Aids for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. This work was also supported by a program of support for women researchers from the Tokyo Medical and Dental University.
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