Immunogenetics

, Volume 62, Issue 9, pp 601–611

Diversity of MHC class I genes in Burmese-origin rhesus macaques

  • Taeko K. Naruse
  • Zhiyong Chen
  • Risa Yanagida
  • Tomoko Yamashita
  • Yusuke Saito
  • Kazuyasu Mori
  • Hirofumi Akari
  • Yasuhiro Yasutomi
  • Masaaki Miyazawa
  • Tetsuro Matano
  • Akinori Kimura
Original Paper

DOI: 10.1007/s00251-010-0462-z

Cite this article as:
Naruse, T.K., Chen, Z., Yanagida, R. et al. Immunogenetics (2010) 62: 601. doi:10.1007/s00251-010-0462-z

Abstract

Rhesus macaques (Macaca mulatta) are widely used in developing a strategy for vaccination against human immunodeficiency virus by using simian immunodeficiency virus infection as a model system. Because the genome diversity of major histocompatibility complex (MHC) is well known to control the immune responsiveness to foreign antigens, MHC loci in Indian- and Chinese-origin macaques used in the experiments have been characterized, and it was revealed that the diversity of MHC in macaques was larger than the human MHC. To further characterize the diversity of Mamu-A and Mamu-B loci, we investigated a total of 73 different sequences of Mamu-A, 83 sequences of Mamu-B, and 15 sequences of Mamu-I cDNAs isolated from Burmese-origin macaques. It was found that there were one to five expressing genes in each locus. Among the Mamu-A, Mamu-B, and Mamu-I sequences, 44 (60.2%), 45 (54.2%), and 8 (53.3%), respectively, were novel, and most of the other known alleles were identical to those reported from Chinese- or Indian-origin macaques, demonstrating a genetic mixture between the geographically distinct populations of present day China and India. In addition, it was found that a Mamu haplotype contained at least two highly transcribed Mamu-A genes, because multiple Mamu-A1 cDNAs were obtained from one haplotype. These findings further revealed the diversity and complexity of MHC locus in the rhesus macaques.

Keywords

Rhesus macaque MHC Mamu-A Mamu-B Mamu-I Haplotype 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Taeko K. Naruse
    • 1
  • Zhiyong Chen
    • 1
  • Risa Yanagida
    • 2
  • Tomoko Yamashita
    • 1
  • Yusuke Saito
    • 2
  • Kazuyasu Mori
    • 3
  • Hirofumi Akari
    • 4
  • Yasuhiro Yasutomi
    • 5
  • Masaaki Miyazawa
    • 6
  • Tetsuro Matano
    • 7
  • Akinori Kimura
    • 1
    • 2
  1. 1.Department of Molecular Pathogenesis, Medical Research InstituteTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Laboratory of Genome Diversity, Graduate School of Biomedical ScienceTokyo Medical and Dental UniversityTokyoJapan
  3. 3.AIDS Research Center, National Institute of Infectious DiseasesTokyoJapan
  4. 4.Primate Research InstituteKyoto UniversityInuyamaJapan
  5. 5.Tsukuba Primate Research Center, National Institute of Biomedical InnovationIbarakiJapan
  6. 6.Department of ImmunologyKinki University School of MedicineOsaka-SayamaJapan
  7. 7.International Research Center for Infectious Diseases, The Institute of Medical ScienceThe University of TokyoTokyoJapan

Personalised recommendations