, Volume 62, Issue 6, pp 383–395

Comprehensive analysis and characterization of the TCR α chain sequences in the common marmoset

  • Yoshiki Fujii
  • Takaji Matsutani
  • Kazutaka Kitaura
  • Satsuki Suzuki
  • Tsunetoshi Itoh
  • Tomohiko Takasaki
  • Ryuji Suzuki
  • Ichiro Kurane
Original Paper


The common marmoset (Callithrix jacchus) is useful as a nonhuman primate model of human diseases. Although the marmoset model has great potential for studying autoimmune diseases and immune responses against pathogens, little information is available regarding the genes involved in adaptive immunity. Here, we identified one TCR α constant (TRAC), 46 TRAJ (joining), and 35 TRAV (variable) segments from marmoset cDNA. Marmoset TRAC, TRAJ, and TRAV shared 80%, 68–100%, and 79–98% identity with their human counterparts at the amino acid level, respectively. The amino acid sequences were less conserved in TRAC than in TCRβ chain constant (TRBC). Comparative analysis of TRAV between marmosets and humans showed that the rates of synonymous substitutions per site (dS) were not significantly different between the framework regions (FRs) and complementarity determining regions (CDRs), whereas the rates of nonsynonymous substitutions per site (dN) were significantly lower in the FRs than in CDRs. Interestingly, the dN values of the CDRs were greater for TRBV than TRAV. These results suggested that after the divergence of Catarrhini from Platyrrhini, amino acid substitutions were decreased in the FRs by purifying selection and occurred more frequently in CDRβ than in CDRα by positive selection, probably depending on structural and functional constraints. This study provides not only useful information facilitating the investigation of adaptive immunity using the marmoset model but also new insight into the molecular evolution of the TCR heterodimer in primate species.


Common marmoset T cell receptors Evolution Comparative immunology 

Supplementary material

251_2010_445_MOESM1_ESM.doc (76 kb)
Supplemental Table 1Comparison of rates of synonymous (dS) or nonsynonymous substitutions per site (dN) in FRs and CDRs of TRBV between humans and the Rhesus monkeys (Macaca mulatta). (DOC 76 kb)
251_2010_445_MOESM2_ESM.doc (1 mb)
Supplemental Figure 1Total RNA was separately extracted from seven individual common marmosets (nos. 1–7). PCR amplification was performed with either TRAV8-6s1 (s1) or TRAV8-6s2 (s2) and CjCA3 primer. The cDNA clones encoding TRAV8-6s1 or TRAV8-6s2 segment were used as positive control. (DOC 1056 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yoshiki Fujii
    • 1
    • 2
  • Takaji Matsutani
    • 3
  • Kazutaka Kitaura
    • 1
    • 2
  • Satsuki Suzuki
    • 4
  • Tsunetoshi Itoh
    • 3
  • Tomohiko Takasaki
    • 2
  • Ryuji Suzuki
    • 1
  • Ichiro Kurane
    • 2
  1. 1.Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and RheumatologySagamihara National Hospital, National Hospital OrganizationSagamiharaJapan
  2. 2.Department of Virology INational Institute of Infectious DiseasesTokyoJapan
  3. 3.Division of Immunology and Embryology, Department of Cell BiologyTohoku University School of MedicineSendaiJapan
  4. 4.Section of Biological Science, Research Center for OdontologyNippon Dental University School of Life DentistryTokyoJapan

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