Immunogenetics

, Volume 61, Issue 9, pp 649–655

FcεRIα gene –18483A>C polymorphism affects transcriptional activity through YY1 binding

  • Daniel P. Potaczek
  • Keiko Maeda
  • Qing-Hui Wang
  • Nobuhiro Nakano
  • Shunsuke Kanada
  • Ewa Stepien
  • Agnieszka Branicka
  • Tatsuo Fukai
  • Mutsuko Hara
  • Tomoko Tokura
  • Hideoki Ogawa
  • Anetta Undas
  • Ko Okumura
  • Chiharu Nishiyama
Brief Communication

Abstract

Three frequent genetic polymorphisms in the human high-affinity IgE receptor α-subunit (FcεRIα) were shown to be associated with allergic disorders and/or total serum IgE levels in allergic patients. Two of these were previously demonstrated to affect FcεRIα expression while the third –18483A>C (rs2494262) has not yet been subjected to functional studies. We hypothesized that the –18483A>C variant affects transcriptional activity of the FcεRIα distal promoter in monocytes in which FcεRIα transcription is driven through that regulatory region. Indeed, we confirmed preferential binding of the YY1 transcription factor to the –18483C allele, resulting in lower transcriptional activity when compared with the –18483A allele.

Keywords

FcεRIα Monocytes Polymorphism YY1 FCER1A 

Supplementary material

251_2009_391_MOESM1_ESM.doc (79 kb)
(DOC 80 kb)

References

  1. Bae JS, Kim SH, Ye YM et al (2007) Significant association of FcεRIα promoter polymorphisms with aspirin-intolerant chronic urticaria. J Allergy Clin Immunol 119:449–456PubMedCrossRefGoogle Scholar
  2. Barrett JC, Fry B, Maller J, Daly MJ (2005) Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics 21:263–265PubMedCrossRefGoogle Scholar
  3. Griffioen M, Ouwerkerk IJ, Harten V, Schrier PI (2000) HLA-B locus-specific downregulation in human melanoma requires enhancer A as well as a sequence element located downstream of the transcription initiation site. Immunogenetics 52:121–128PubMedCrossRefGoogle Scholar
  4. Guo J, Casolaro V, Seto E et al (2001) Yin-Yang 1 activates interleukin-4 gene expression in T cells. J Biol Chem 276:48871–48878PubMedCrossRefGoogle Scholar
  5. Hasegawa M, Nishiyama C, Nishiyama M et al (2003a) Regulation of the human FcεRI α-chain distal promoter. J Immunol 170:3732–3738PubMedGoogle Scholar
  6. Hasegawa M, Nishiyama C, Nishiyama M et al (2003b) A novel -66 T/C polymorphism in FcεRI α-chain promoter affecting the transcription activity: possible relationship to allergic diseases. J Immunol 171:1927–1933PubMedGoogle Scholar
  7. Javahery R, Khachi A, Lo K, Zenzie-Gregory B, Smale ST (1994) DNA sequence requirements for transcriptional initiator activity in mammalian cells. Mol Cell Biol 14:116–127PubMedGoogle Scholar
  8. Kanada S, Nakano N, Potaczek DP et al (2008) Two different transcription factors discriminate the -315C>T-polymorphism of the FcεRI α-chain gene: binding of Sp1 to -315C and of a high mobility group-related molecule to -315 T. J Immunol 180:8204–8210PubMedGoogle Scholar
  9. Maeda K, Nishiyama C, Tokura T et al (2006) FOG-1 represses GATA-1-dependent FcepsilonRI beta-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice. Blood 108:262–269PubMedCrossRefGoogle Scholar
  10. Mordvinov VA, Schwenger GT, Fournier R et al (1999) Binding of YY1 and Oct1 to a novel element that downregulates expression of IL-5 in human T cells. J Allergy Clin Immunol 103:1125–1135PubMedCrossRefGoogle Scholar
  11. Nishiyama C, Hasegawa M, Nishiyama M et al (2001) Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation. Biochem Biophys Res Commun 284:1056–1064PubMedCrossRefGoogle Scholar
  12. Nishiyama C, Hasegawa M, Nishiyama M et al (2002) Regulation of human FcεRI α-chain gene expression by multiple transcription factors. J Immunol 168:4546–4552PubMedGoogle Scholar
  13. Nishiyama C, Akizawa Y, Nishiyama M et al (2004) Polymorphisms in the FcεRIβ promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between FcεRIβ and atopy. J Immunol 173:6458–6464PubMedGoogle Scholar
  14. Potaczek DP, Sanak M, Mastalerz L et al (2006) The α-chain of high-affinity receptor for IgE (FcεRIα) gene polymorphisms and serum IgE levels. Allergy 61:1230–1233PubMedCrossRefGoogle Scholar
  15. Potaczek DP, Sanak M, Mastalerz L, Milewski M, Gawlewicz-Mroczka A, Szczeklik A (2007a) Genetic polymorphisms of the novel FCER1A gene region: relation to total serum IgE levels. Ann Allergy Asthma Immunol 98:500–501PubMedCrossRefGoogle Scholar
  16. Potaczek DP, Sanak M, Szczeklik A (2007b) Additive association between FCER1A and FCER1B genetic polymorphisms and total serum IgE levels. Allergy 62:1095–1096PubMedCrossRefGoogle Scholar
  17. Potaczek DP, Nishiyama C, Sanak M, Szczeklik A, Okumura K, Ogawa H (2008) FCER1A gene exon 1A polymorphisms in Japanese and Polish subjects—a comparative analysis of haplotypes. Allergy 63:626–627PubMedCrossRefGoogle Scholar
  18. Shikanai T, Silverman ES, Morse BW, Lilly CM, Inoue H, Drazen JM (2002) Sequence variants in the FcεRI alpha chain gene. J Appl Physiol 93:37–41PubMedGoogle Scholar
  19. Shrivastava A, Calame K (1994) An analysis of genes regulated by the multi-functional transcriptional regulator Yin Yang-1. Nucleic Acids Res 22:5151–5155PubMedCrossRefGoogle Scholar
  20. Silverman ES, Palmer LJ, Subramaniam V et al (2004) Transforming growth factor-β1 promoter polymorphism C–509 T is associated with asthma. Am J Respir Crit Care Med 169:214–219PubMedCrossRefGoogle Scholar
  21. Tregouet DA, Garelle V (2007) A new JAVA interface implementation of THESIAS: testing haplotypes effects in association studies. Bioinformatics 23:1038–1039PubMedCrossRefGoogle Scholar
  22. Wang QH, Nishiyama C, Nakano N et al (2008) Suppresive effect of Elf-1 on FcεRI α-chain expression in primary mast cells. Immunogenetics 60:557–563PubMedCrossRefGoogle Scholar
  23. Zhang M, Murphy RF, Agrawal DK (2007) Decoding IgE Fc receptors. Immunol Res 37:1–16PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Daniel P. Potaczek
    • 1
  • Keiko Maeda
    • 1
  • Qing-Hui Wang
    • 1
    • 2
  • Nobuhiro Nakano
    • 1
  • Shunsuke Kanada
    • 1
    • 3
  • Ewa Stepien
    • 4
  • Agnieszka Branicka
    • 4
  • Tatsuo Fukai
    • 1
    • 5
  • Mutsuko Hara
    • 1
  • Tomoko Tokura
    • 1
  • Hideoki Ogawa
    • 1
  • Anetta Undas
    • 6
  • Ko Okumura
    • 1
    • 3
  • Chiharu Nishiyama
    • 1
  1. 1.Atopy (Allergy) Research CenterJuntendo University School of MedicineTokyoJapan
  2. 2.Department of Immunology, College of Basic Medical SciencesChina Medical UniversityShenyangChina
  3. 3.Department of ImmunologyJuntendo University School of MedicineTokyoJapan
  4. 4.John Paul II HospitalKrakowPoland
  5. 5.Department of DermatologyJuntendo University School of MedicineTokyoJapan
  6. 6.Institute of CardiologyJagiellonian University School of MedicineKrakowPoland

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