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Immunogenetics

, Volume 57, Issue 3–4, pp 219–225 | Cite as

TRAV gene usage in pig T-cell receptor alpha cDNA

  • Ryuji Yamamoto
  • Hirohide Uenishi
  • Hiromi Hatsuse
  • Eimei Sato
  • Takashi Awata
  • Hiroshi Yasue
  • Yohtaroh Takagaki
Original Paper

Abstract

Pig (Sus scrofa) TRA clones were isolated from cDNA libraries of total RNA from two different sources, the thymus of a 1-month-old LW strain pig and the peripheral blood lymphocytes of a 5-month-old Clawn strain pig. Among 103 complete TRA cDNA clones from both sources, 33 different TRAV genes were identified. By comparing their sequence identities against one another, these pig TRAV genes were grouped into 20 subgroups, including 13 subgroups, each containing only a single member. All of these pig subgroups gave corresponding human and mouse functional counterparts, suggesting their functional commonality. An exception was the Va01 gene segment, which lacked a functional human counterpart. The present report provides groundwork for studies on pig TRA expression.

Keywords

T-cell receptor alpha (TRA) chain Pig 

Notes

Acknowledgements

We thank Tomiji Akita for care of pigs. This work was supported by grants from National Institute of Agrobiological Sciences, Japan.

Supplementary material

251_2005_779_ESM_supp.pdf (72 kb)
(PDF 72 KB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ryuji Yamamoto
    • 1
    • 2
    • 3
  • Hirohide Uenishi
    • 1
  • Hiromi Hatsuse
    • 2
  • Eimei Sato
    • 3
  • Takashi Awata
    • 1
  • Hiroshi Yasue
    • 1
  • Yohtaroh Takagaki
    • 2
  1. 1.Genome Research DepartmentNational Institute of Agrobiological SciencesTsukubaJapan
  2. 2.Department of Molecular Genetics, Graduate School of Medical SciencesKitasato UniversitySagamiharaJapan
  3. 3.Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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