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Immunogenetics

, Volume 57, Issue 8, pp 601–606 | Cite as

Serological and molecular diversity in the cattle MHC class I region

  • Shirley A. EllisEmail author
  • W. Ivan Morrison
  • Niall D. MacHugh
  • James Birch
  • Alison Burrells
  • Michael J. Stear
Original Paper

Abstract

Information on major histocompatibility complex (MHC) diversity in cattle is important to aid our understanding of immune responses and may contribute to maintenance of healthy cattle populations. Equally, understanding the mechanisms involved in generating this diversity may shed light on the complex nature of mammalian MHC evolution. The aim of this study was to assess molecular and serological variation within cattle MHC class I molecules and to study the mechanisms generating diversity. To address this aim, sequence variation was examined in 12 serologically assigned alleles from three putative loci and correlated with monoclonal antibody (mAb) binding data. The results demonstrate that both alloantisera and mAbs often fail to distinguish gene products that differ by a significant number of amino acids. Conversely, some mAbs could distinguish alleles differing by only one or two amino acids. Examination of the sequences demonstrates sharing of motifs between alleles, some encoded at distinct loci, supporting the occurrence of interlocus recombination within the cattle MHC class I region. The implications of this for MHC sequence diversity, and functional capability, are discussed.

Keywords

MHC class I Cattle Polymorphism 

Notes

Acknowledgements

This work was supported by the Biological Sciences and Biotechnology Research Council, UK. We would like to thank Karen Staines for technical assistance. Experiments carried out in this study comply with the UK law.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Shirley A. Ellis
    • 1
    Email author
  • W. Ivan Morrison
    • 2
  • Niall D. MacHugh
    • 2
  • James Birch
    • 1
  • Alison Burrells
    • 2
  • Michael J. Stear
    • 3
  1. 1.Institute for Animal HealthNewburyUK
  2. 2.The Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary StudiesThe University of EdinburghMidlothianUK
  3. 3.Division of Animal Production and Comparative MedicineInstitute of Comparative Medicine, Faculty of Veterinary Medicine, Glasgow UniversityGlasgowUK

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