HLA-G and IL-10 in serum in relation to HLA-G genotype and polymorphisms
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The expression and importance of the non-classical human leukocyte antigen (HLA) class Ib gene, HLA-G, at the feto-maternal interface have been recognized. The HLA-G molecule is almost monomorphic and expressed in both membrane-bound and soluble isoforms. It has been shown to inhibit NK-mediated cell lysis and influence cytokine expression. Recently, a possible boarder immunoregulatory function of HLA-G also in adult life has been recognized. HLA-G gene polymorphism has been linked to differences in gene expression profile of alternatively spliced HLA-G transcripts and levels of specific HLA-G mRNA isoforms. On this background it is of general interest to further elucidate any associations between HLA-G polymorphism and protein expression. We have HLA-G genotyped 85 individuals attending IVF treatment, and further studied sHLA-G1/HLA-G5 and interleukin-10 (IL-10) in serum samples. In 21% of the serum samples sHLA-G1/HLA-G5 could be detected. There was no correlation between sHLA-G1/HLA-G5 and IL-10 concentrations in serum. Soluble HLA-G1/HLA-G5 was not detected in any samples homozygous for a 14-bp insertion polymorphism in exon 8 of the 3′-untranslated region (3′UTR) of the HLA-G gene (P=0.03; Fisher’s exact test). Polymorphisms in the 5′-upstream regulatory region (5′URR) of the HLA-G gene were also studied. In conclusion, this study indicates that polymorphisms in the 3′UTR and the 5′URR of the HLA-G gene may influence the expression of sHLA-G of possible importance in pathological pregnancies and also in organ transplantation.
KeywordsSoluble HLA-G MHC Polymorphism IL-10 Immunoregulation
This study was supported by The Pharmacy Foundation of 1991, The Copenhagen Hospital Corporation, The A.P. Møller Foundation for the Advancement of Medical Science, The Plasmid Foundation and The Danish Medical Association Research Fund.
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