Advertisement

Immunogenetics

, Volume 54, Issue 9, pp 621–634 | Cite as

Cloning of S4D-SRCRB, a new soluble member of the group B scavenger receptor cysteine-rich family (SRCR-SF) mapping to human Chromosome 7q11.23

  • Olga Padilla
  • Miguel Pujana
  • Agustí López-de la Iglesia
  • Idoia Gimferrer
  • Mònica Arman
  • Josep Vilà
  • Lourdes Places
  • Jordi Vives
  • Xavier Estivill
  • Francisco Lozano
Original Paper

Abstract.

The scavenger receptor cysteine-rich superfamily (SRCR-SF) is a highly conserved group of membrane and/or secreted proteins related to the innate and adaptive immune system. Here, we report the cloning of the gene encoding human S4D-SRCRB, a novel soluble member of the SRCR-SF, which is composed of four group B SRCR domains separated by Pro-, Ser- and Thr-rich polypeptides. The longest cDNA sequence found is 2,806 bp in length and encodes a mature protein of 528 aa, with a predicted molecular mass of Mr 55,600. The S4D-SRCRB gene is located at Chromosome 7q11.23, telomeric to the Williams–Beuren syndrome deletion. It extends over 20 kb and consists of 11 exons, with each SRCR domain being encoded by a single exon. Northern blot analysis indicated that S4D-SRCRB has a broad tissue distribution and is expressed as two major mRNA species: one of 2.8 kb, with a restricted tissue expression pattern (mainly kidney and placenta), and another of 1.5 kb, with a broader distribution. A similar mRNA expression pattern was observed during the analysis of several tumor cell lines. The highest degree of similarity found between S4D-SRCRB and other group B SRCR-SF members was with human DMBT1 (a mosaic protein composed of fourteen SRCR domains, which is involved in innate defense and epithelia polarization) and chicken 18-B (a turpentine-induced soluble acute-phase protein composed of four SRCR domains). Our data indicate that S4D-SRCRB constitutes a novel SRCR-SF member, which could be involved in basic homeostatic functions such as innate host defense.

S4D-SRCRB SRCR superfamily Human Chromosome 7q11.23 Williams–Beuren syndrome deletion Innate defense 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Olga Padilla
    • 1
  • Miguel Pujana
    • 2
  • Agustí López-de la Iglesia
    • 1
  • Idoia Gimferrer
    • 1
  • Mònica Arman
    • 1
  • Josep Vilà
    • 1
  • Lourdes Places
    • 1
  • Jordi Vives
    • 1
  • Xavier Estivill
    • 2
  • Francisco Lozano
    • 1
  1. 1.Institut Clínic d'Infeccions i Immunologia (ICII), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Villarroel 170, 08036 Barcelona, Spain
  2. 2.Centre de Genètica Mèdica i Molecular, Institut de Recerca Oncològica (IRO), and Genes and Disease Program, Centre de Regulació Genòmica (CRG), Autovia de Castelldefels Km. 2.7, 08907 L'Hospitalet de Llobregat, Spain

Personalised recommendations