Immunogenetics

, Volume 54, Issue 6, pp 394–402 | Cite as

Mouse novel Ly9: a new member of the expanding CD150 (SLAM) family of leukocyte cell-surface receptors

  • Victoria Tovar
  • Juana del Valle
  • Nuria Zapater
  • Margarita Martin
  • Xavier Romero
  • Pilar Pizcueta
  • Jaime Bosch
  • Cox Terhorst
  • Pablo Engel
Original Paper

Abstract.

Human CS1, also known as novel Ly9, 19A24, or CRACC, is a member of the immunoglobulin gene superfamily (IgSF) expressed on natural killer cells and other leukocytes. Here we describe the cloning of the mouse homologue of this gene. The mouse novel Ly9 gene is shown to encode a transmembrane protein composed of two extracellular immunoglobulin-like domains, a transmembrane region and an 88-amino acid cytoplasmic domain. Mouse novel Ly9 is structurally similar to the extracellular domains of CD84 and CD229 (Ly9). Both mouse and human novel Ly9 genes mapped close to the CD229 gene in a region where other members of the CD150 family have also been mapped, and analysis of their genomic sequences showed that they have an identical intron/exon organization. Northern blot analysis revealed that the expression of mouse and human novel Ly9 was predominantly restricted to hematopoietic tissues, with the exception of testis. Here we show that SAP (SH2D1A), an adapter protein responsible for the X-linked lymphoproliferative disease, binds to the phosphorylated cytoplasmic tail of human but not mouse novel Ly9. Taken together, these data indicate that mouse novel Ly9 is a new member of the expanding CD150 family of cell surface receptors.

Cell surface molecule Chromosome 1 Immunoglobulin superfamily CD150 SLAM-associated protein 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Victoria Tovar
    • 1
  • Juana del Valle
    • 1
  • Nuria Zapater
    • 1
  • Margarita Martin
    • 1
  • Xavier Romero
    • 1
  • Pilar Pizcueta
    • 2
  • Jaime Bosch
    • 2
  • Cox Terhorst
    • 3
  • Pablo Engel
    • 1
  1. 1.Immunology Unit, Department of Cellular Biology and Pathology, Medical School, University of Barcelona, Barcelona 08036, Spain
  2. 2.Liver Unit, Institut Malalties Digestives, Hospital Clínic, Barcelona 08036, Spain
  3. 3.Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA

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