European Biophysics Journal

, Volume 30, Issue 6, pp 457–462 | Cite as

HYDROMIC: prediction of hydrodynamic properties of rigid macromolecular structures obtained from electron microscopy images

  •  J. García de la Torre
  •  O. Llorca
  •  J. Carrascosa
  •  J. Valpuesta
Article

Abstract.

We have developed a procedure for the prediction of hydrodynamic coefficients and other solution properties of macromolecules and macromolecular complexes whose volumes have been generated from electron microscopy images. Starting from the structural files generated in the three-dimensional reconstructions of such molecules, it is possible to construct a hydrodynamic model for which the solution properties can be calculated. We have written a computer program, HYDROMIC, that implements all the stages of the calculation. The use of this procedure is illustrated with a calculation of the solution properties of the volume of the cytosolic chaperonin CCT, obtained from cryoelectron microscopy images.

HYDROMIC Hydrodynamic coefficients Solution properties Macromolecules Electron microscopy 

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Copyright information

© EBSA 2001

Authors and Affiliations

  •  J. García de la Torre
    • 1
  •  O. Llorca
    • 2
  •  J. Carrascosa
    • 2
  •  J. Valpuesta
    • 2
  1. 1.Departamento de Química Física, Universidad de Murcia, 30071 Murcia, Spain
  2. 2.Centro Nacional de Biotecnología, C.S.I.C., Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain

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